Tiparp Protein

Catalog No. ABIN7939640

This Recombinant Tiparp protein is produced in Baculovirus infected Insect Cells.

Quick Overview for Tiparp Protein (ABIN7939640)

Target: Tiparp (TCDD-Inducible Poly(ADP-Ribose) Polymerase (Tiparp))

Protein Type: Recombinant

Origin: Human

Source: Baculovirus infected Insect Cells

Application: Activity Assay (AcA), Functional Studies (Func), Screening Assay (ScA)

Product Details

Purpose: Recombinant PARP7 protein

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Recombinant PARP7 protein is supplied in 25 mM HEPES pH 7.5, 300 mM NaCl, 20 % glycerol, 0.04 % Triton X-100, 0.5 mM TCEP.

Handling Advice: Avoid repeated freeze/thaw cycles,keep on ice when not in storage

Storage: -80 °C

Storage Comment: Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

Target Details

Target: Tiparp (TCDD-Inducible Poly(ADP-Ribose) Polymerase (Tiparp))

Alternative Name: PARP7

Background:

Protein Background: PARP7(Protein mono-ADP-ribosyltransferase TIPARP), also known as TiPARP, is a mono ADPribosyltransferase belonging to the Poly-ADP-ribose polymerase (PARP) family. Unlike some PARPs involved in DNA repair, PARP7's primary functions are linked to regulating gene expression and the immune response, particularly the type I interferon signaling pathway. It is induced by the transcription factor AHR (aryl hydrocarbon receptor) and acts as a negative feedback regulator. PARP7 modifies itself and other target proteins with a single ADP-ribose unit (MARylation), which influences their activity and stability. By MARylating key transcription factors like AHR and IRF3/7, it dampens inflammatory and interferon responses, thereby maintaining immune homeostasis. Its role as a checkpoint in anti-tumor immunity has made it a emerging target for cancer immunotherapy, where its inhibition is sought to enhance the body's innate immune attack against cancer cells.

Short Description: Recombinant PARP7 protein was expressed in a baculovirus expression system as the full length protein (accession number NP_056323.2) with an N-terminal FLAG tag. The molecular weight of PARP7 is 77.5 kDa.

Aliases: Protein mono-ADP-ribosyltransferase TIPARP, TiPARP, ARTD14, DDF1, RM1, PART14

Pathways: Platelet-derived growth Factor Receptor Signaling