TOP2B Protein

Catalog No. ABIN7939648

Recombinant TOP2B protein expressed in Escherichia coli (E. coli).

Quick Overview for TOP2B Protein (ABIN7939648)

Target: TOP2B (Topoisomerase (DNA) II beta 180kDa (TOP2B))

Protein Type: Recombinant

Origin: Human

Source: Escherichia coli (E. coli)

Application: Activity Assay (AcA), Functional Studies (Func), Screening Assay (ScA)

Product Details

Purpose: Recombinant TOP2B protein

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Recombinant TOP2B protein is supplied in 25 mM HEPES pH 7.5, 500 mM NaCl,500 mM KCl,10 % glycerol, 1 mM MgCl2,0.04 % Triton X-100, 0.5 mM TCEP.

Handling Advice: Avoid repeated freeze/thaw cycles,keep on ice when not in storage

Storage: -80 °C

Storage Comment: Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

Target Details

Target: TOP2B (Topoisomerase (DNA) II beta 180kDa (TOP2B))

Alternative Name: TOP2B

Background:

Protein Background: TOP2B (DNA topoisomerase 2-beta ) is key decatenating enzyme that alters DNA topology by binding to two double-stranded DNA molecules, generating a double-stranded break in one of the strands, passing the intact strand through the broken strand, and religating the broken strand. Plays a role in B-cell differentiation.

Short Description: Recombinant TOP2B protein was expressed in a baculovirus expression system as the full length protein (accession number NP_001059.2) with an N-terminal FLAG tag. The molecular weight of TOP2B is 183.93 kDa.

Aliases: DNA topoisomerase 2-beta, Top2beta, TOPIIB, Topoisomerase II, BILU