TRMT61A Protein

Catalog No. ABIN7939661

Recombinant TRMT61A protein expressed in Escherichia coli (E. coli).

Quick Overview for TRMT61A Protein (ABIN7939661)

Target: TRMT61A (tRNA Methyltransferase 61 Homolog A (TRMT61A))

Protein Type: Recombinant

Origin: Human

Source: Escherichia coli (E. coli)

Application: Activity Assay (AcA), Functional Studies (Func), Screening Assay (ScA)

Product Details

Purpose: Recombinant TRMT61A protein

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Recombinant TRMT61A protein is supplied in 25 mM Tris-HCl pH 8.0, 300 mM NaCl, and 5 % glycerol.

Handling Advice: Avoid repeated freeze/thaw cycles,keep on ice when not in storage

Storage: -80 °C

Storage Comment: Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

Target Details

Target: TRMT61A (tRNA Methyltransferase 61 Homolog A (TRMT61A))

Alternative Name: TRMT61A

Background:

Protein Background: TRMT61A (tRNA (adenine(58)-N(1))-methyltransferase catalytic subunit TRMT61A) is a catalytic subunit of tRNA (adenine-N1-)-methyltransferase, which catalyzes the formation of N1-methyladenine at position 58 (m1A58) in initiator methionyl-tRNA .TRMT61A is also a catalytic subunit of mRNA N1-methyltransferase complex, which mediates methylation of adenosine residues at the N1 position of a small subset of mRNAs: N1 methylation takes place in tRNA T-loop-like structures of mRNAs and is only present at low stoichiometries.

Short Description: Recombinant TRMT61A protein that includes amino acids 1-289 of human TRMT61A protein (accession number NP_689520.2) was expressed was expressed in E. coli cells with an N-terminal 6x His tag. The molecular weight of the protein is 33.18 kDa.

Aliases: TRNA Methyltransferase 61A, tRNA (adenine (58)-N(1)-Methyltransferase catalytic Subunit TRM61A