Ubiquitin Protein (Ub) (AA 1-76)

Catalog No. ABIN7939667

Recombinant Ubiquitin protein expressed in Escherichia coli (E. coli).

Quick Overview for Ubiquitin Protein (Ub) (AA 1-76) (ABIN7939667)

Target: Ubiquitin (Ub)

Protein Type: Recombinant

Origin: Human

Source: Escherichia coli (E. coli)

Application: Activity Assay (AcA), Functional Studies (Func), Screening Assay (ScA)

Product Details

Protein Characteristics: AA 1-76

Purpose: Recombinant Ubiquitin (1-76) protein

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Recombinant Ubiquitin (1-76) protein is supplied in 25 mM Tris pH 8.0, 150 mM NaCl, 20 % glycerol, 0.5 mM TCEP.

Handling Advice: Avoid repeated freeze/thaw cycles,keep on ice when not in storage

Storage: -80 °C

Storage Comment: Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

Target Details

Target: Ubiquitin (Ub)

Alternative Name: Ubiquitin

Background:

Protein Background: Ubiquitin exists either covalently attached to another protein, or free (unanchored). When covalently bound, it is conjugated to target proteins via an isopeptide bond either as a monomer (monoubiquitin), a polymer linked via different Lys residues of the ubiquitin (polyubiquitin chains) or a linear polymer linked via the initiator Met of the ubiquitin (linear polyubiquitin chains). Polyubiquitin chains, when attached to a target protein, have different functions depending on the Lys residue of the ubiquitin that is linked. Linear polymer chains formed via attachment by the initiator Met lead to cell signaling. Ubiquitin is usually conjugated to Lys residues of target proteins, however, in rare cases, conjugation to Cys or Ser residues has been observed. When polyubiquitin is free (unanchored-polyubiquitin), it also has distinct roles, such as in activation of protein kinases, and in signaling.

Short Description: Recombinant Ubiquitin (1-76) protein that includes amino acids 1-76 of human UBC protein (accession number NP_066289.3) was expressed in E. coli and without any tag with a molecular weight of 8.56 kDa. The purity of the protein is ≥ 95 % by SDS-PAGE.

Pathways: Mitotic G1-G1/S Phases, Ubiquitin Proteasome Pathway