UBE2B Protein

Catalog No. ABIN7939676

This Recombinant UBE2B protein is produced in Escherichia coli (E. coli).

Quick Overview for UBE2B Protein (ABIN7939676)

Target: UBE2B (Ubiquitin-Conjugating Enzyme E2B (UBE2B))

Protein Type: Recombinant

Origin: Human

Source: Escherichia coli (E. coli)

Application: Activity Assay (AcA), Functional Studies (Func), Screening Assay (ScA)

Product Details

Purpose: Recombinant UBE2B protein

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Recombinant UBE2B protein is supplied in 25 mM Tris 8.0, 300 mM NaCl, 20 % glycerol, 0.5 mM TCEP.

Handling Advice: Avoid repeated freeze/thaw cycles,keep on ice when not in storage

Storage: -80 °C

Storage Comment: Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

Target Details

Target: UBE2B (Ubiquitin-Conjugating Enzyme E2B (UBE2B))

Alternative Name: UBE2B

Background:

Protein Background: UBE2B (Ubiquitin-conjugating enzyme E2 B) also known as E2-17 kDa, HHR6B, HR6B, RAD6B and UBC2. UBE2B is a member of the E2 ubiquitin-conjugating enzyme family which is required for post-replicative DNA damage repair and plays an important role in various cellular processes. UBE2B can accept ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In association with the E3 enzyme BRE1 (RNF20 and/or RNF40), it plays a role in transcription regulation by catalyzing the monoubiquitination of histone H2B at 'Lys-120' to form H2BK120ub1. H2BK120ub1 gives a specific tag for epigenetic transcriptional activation, elongation by RNA polymerase II, telomeric silencing, and is also a prerequisite for H3K4me and H3K79me formation. In vitro catalyzes 'Lys-11'-, as well as 'Lys-48'- and 'Lys-63'-linked polyubiquitination. Required for postreplication repair of UV-damaged DNA. Associates to the E3 ligase RAD18 to form the UBE2B-RAD18 ubiquitin ligase complex involved in mono-ubiquitination of DNA-associated PCNA on 'Lys-164'. May be involved in neurite outgrowth.

Short Description: Recombinant UBE2B protein that includes full length of human UBE2B protein (accession number NP_003328.1) was expressed in E. coli and contains an N-terminal His tag with a molecular weight of 19.48 kDa. The purity of the protein is ≥ 95 % by SDS-PAGE.

Aliases: Ubiquitin conjugating enzyme E2 B, E2-17 kDa, HHR6B, RAD6B, UBC2