UBE2D4 Protein
Quick Overview for UBE2D4 Protein (ABIN7939687)
Target
See all UBE2D4 ProteinsProtein Type
Origin
Source
Application
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Purpose
- Recombinant UBE2D4 protein
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Ubiquitin-Conjugating Enzyme E2D 4 (UBE2D4) (AA 1-147) protein (His tag)
custom-made UBE2D4 Origin: Human Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
Ubiquitin-Conjugating Enzyme E2D 4 (UBE2D4) (AA 1-147) protein (GST tag)UBE2D4 Origin: Human Host: Wheat germ Recombinant ELISA, WB, AA, AP
Ubiquitin-Conjugating Enzyme E2D 4 (UBE2D4) (AA 1-147) protein (His-IF2DI Tag)UBE2D4 Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 90 % as determined by SDS-PAGE. ELISA, WB
Ubiquitin-Conjugating Enzyme E2D 4 (UBE2D4) (AA 1-147) protein (His tag)UBE2D4 Origin: Human Host: Escherichia coli (E. coli) Recombinant > 95 % SDS
Ubiquitin-Conjugating Enzyme E2D 4 (UBE2D4) protein (Myc-DYKDDDDK Tag)UBE2D4 Origin: Human Host: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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Application Notes
- Optimal working dilution should be determined by the investigator.
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Restrictions
- For Research Use only
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Format
- Liquid
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Buffer
- Recombinant UBE2D4 protein is supplied in 25 mM Tris 7.4, 300 mM NaCl, 20 % glycerol, 0.5 mM TCEP.
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Handling Advice
- Avoid repeated freeze/thaw cycles,keep on ice when not in storage
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Storage
- -80 °C
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Storage Comment
- Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.
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- UBE2D4 (Ubiquitin-Conjugating Enzyme E2D 4 (UBE2D4))
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Alternative Name
- UBE2D4
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Background
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Protein Background: UBE2D4 (Ubiquitin-conjugating enzyme E2 D4) also known as HBUCE1. UBE2D4 is a member of the E2 ubiquitin-conjugating enzyme family which is required for post-replicative DNA damage repair and plays an important role in various cellular processes. UBE2D4 can accept ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In vitro able to promote polyubiquitination using all 7 ubiquitin Lys residues but may prefer 'Lys-11' and 'Lys-48'-linked polyubiquitination.
Short Description: Recombinant UBE2D4 protein that includes full length of human UBE2D4 protein (accession number NP_057067.1) was expressed in E. coli and contains an N-terminal His tag with a molecular weight of 18.81 kDa. The purity of the protein is ≥ 95 % by SDS-PAGE.
Aliases: Ubiquitin conjugating enzyme E2 D4, HBUCE1, UBCH5D
Target
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