UBE2E1 Protein
Quick Overview for UBE2E1 Protein (ABIN7939689)
Target
See all UBE2E1 ProteinsProtein Type
Origin
Source
Application
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Purpose
- Recombinant UBE2E1 protein
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Ubiquitin-Conjugating Enzyme E2E 1 (UBE2E1) (AA 1-193) protein (GST tag)
UBE2E1 Origin: Human Host: Wheat germ Recombinant WB, ELISA, AA, AP
Ubiquitin-Conjugating Enzyme E2E 1 (UBE2E1) (AA 1-193) protein (His tag)UBE2E1 Origin: Human Host: Escherichia coli (E. coli) Recombinant > 90 % SDS
Ubiquitin-Conjugating Enzyme E2E 1 (UBE2E1) (AA 1-193) protein (His-IF2DI Tag)UBE2E1 Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 90 % as determined by SDS-PAGE. WB, ELISA
Ubiquitin-Conjugating Enzyme E2E 1 (UBE2E1) (Transcript Variant 2) protein (Myc-DYKDDDDK Tag)UBE2E1 Origin: Human Host: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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Application Notes
- Optimal working dilution should be determined by the investigator.
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Restrictions
- For Research Use only
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Format
- Liquid
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Buffer
- Recombinant UBE2E1 protein is supplied in 25 mM Tris 8.0, 300 mM NaCl, 20 % glycerol, 0.5 mM TCEP.
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Handling Advice
- Avoid repeated freeze/thaw cycles,keep on ice when not in storage
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Storage
- -80 °C
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Storage Comment
- Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.
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- UBE2E1 (Ubiquitin-Conjugating Enzyme E2E 1 (UBE2E1))
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Alternative Name
- UBE2E1
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Background
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Protein Background: UBE2E1 (Ubiquitin-conjugating enzyme E2 E1) also known as UBCH6. UBE2E1 is a member of the E2 ubiquitin-conjugating enzyme family which is required for post-replicative DNA damage repair and plays an important role in various cellular processes. UBE2E1 can accept ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. Catalyzes the covalent attachment of ISG15 to other proteins. Mediates the selective degradation of short-lived and abnormal proteins. In vitro also catalyzes 'Lys-48'-linked polyubiquitination.
Short Description: Recombinant UBE2E1 protein that includes full length of human UBE2E1 protein (accession number NP_003332.1) was expressed in E. coli and contains an N-terminal His tag with a molecular weight of 23.57 kDa. Thepurity of the protein is ≥ 95 % by SDS-PAGE.
Aliases: Ubiquitin conjugating enzyme E2 E1, UBCH6
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Pathways
- Ubiquitin Proteasome Pathway
Target
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