UBE2F Protein
Quick Overview for UBE2F Protein (ABIN7939694)
Target
See all UBE2F ProteinsProtein Type
Origin
Source
Application
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Purpose
- Recombinant UBE2F protein
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Ubiquitin-Conjugating Enzyme E2F (UBE2F) (AA 1-185) protein (His tag)
custom-made UBE2F Origin: Human Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
Ubiquitin-Conjugating Enzyme E2F (UBE2F) (AA 1-185) protein (GST tag)UBE2F Origin: Human Host: Wheat germ Recombinant WB, ELISA, AA, AP
Ubiquitin-Conjugating Enzyme E2F (UBE2F) (AA 1-185) protein (His-IF2DI Tag)UBE2F Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 90 % as determined by SDS-PAGE. WB, ELISA
Ubiquitin-Conjugating Enzyme E2F (UBE2F) protein (Myc-DYKDDDDK Tag)UBE2F Origin: Human Host: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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Application Notes
- Optimal working dilution should be determined by the investigator.
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Restrictions
- For Research Use only
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Format
- Liquid
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Buffer
- Recombinant UBE2F protein is supplied in 25 mM Tris 8.0, 300 mM NaCl, 20 % glycerol, 0.5 mM TCEP.
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Handling Advice
- Avoid repeated freeze/thaw cycles,keep on ice when not in storage
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Storage
- -80 °C
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Storage Comment
- Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.
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- UBE2F (Ubiquitin-Conjugating Enzyme E2F (UBE2F))
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Alternative Name
- UBE2F
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Background
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Protein Background: UBE2F (NEDD8-conjugating enzyme UBE2F) also known as NCE2. UBE2F is a member of the E2 ubiquitin-conjugating enzyme family which is required for post-replicative DNA damage repair and plays an important role in various cellular processes. UBE2F can accept the ubiquitin-like protein NEDD8 from the UBA3-NAE1 E1 complex and catalyzes its covalent attachment to other proteins. The specific interaction with the E3 ubiquitin ligase RBX2, but not RBX1, suggests that the RBX2-UBE2F complex neddylates specific target proteins, such as CUL5.
Short Description: Recombinant UBE2F protein that includes full length of human UBE2F protein (accession number NP_001265234.1) was expressed in E. coli and contains an N-terminal His tag with a molecular weight of 23.24 kDa. The purity of the protein is ≥ 95 % by SDS-PAGE.
Aliases: Ubiquitin Conjugating Enzyme E2 F, NEDD8 conjugating enzyme, NCE2
Target
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