UBE2L3 Protein
Quick Overview for UBE2L3 Protein (ABIN7939707)
Target
See all UBE2L3 ProteinsProtein Type
Origin
Source
Application
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Purpose
- Recombinant UBE2L3 protein
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Ubiquitin-Conjugating Enzyme E2L 3 (UBE2L3) (AA 1-154) protein (His tag)
custom-made UBE2L3 Origin: Human Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
Ubiquitin-Conjugating Enzyme E2L 3 (UBE2L3) (AA 1-154) protein (GST tag)UBE2L3 Origin: Human Host: Wheat germ Recombinant WB, ELISA, AA, AP
Ubiquitin-Conjugating Enzyme E2L 3 (UBE2L3) (AA 1-154) proteinUBE2L3 Origin: Human Host: Escherichia coli (E. coli) Recombinant > 95 % SDS
Ubiquitin-Conjugating Enzyme E2L 3 (UBE2L3) (Transcript Variant 1) protein (Myc-DYKDDDDK Tag)UBE2L3 Origin: Human Host: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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Application Notes
- Optimal working dilution should be determined by the investigator.
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Restrictions
- For Research Use only
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Format
- Liquid
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Buffer
- Recombinant UBE2L3 protein is supplied in 25 mM Tris 8.0, 300 mM NaCl, 20 % glycerol, 0.5 mM TCEP.
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Handling Advice
- Avoid repeated freeze/thaw cycles,keep on ice when not in storage
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Storage
- -80 °C
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Storage Comment
- Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.
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- UBE2L3 (Ubiquitin-Conjugating Enzyme E2L 3 (UBE2L3))
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Alternative Name
- UBE2L3
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Background
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Protein Background: UBE2L3 (Ubiquitin-conjugating enzyme E2 L3) also known as E2-F1, L-UBC, UBCH7 and UbcM4. UBE2L3 that specifically acts with HECT-type and RBR family E3 ubiquitin-protein ligases. It has activity with the RBR family E3 enzymes, such as PRKN and ARIH1, that function like RING-HECT hybrids. Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In vitro catalyzes 'Lys-11'-linked polyubiquitination. Involved in the selective degradation of short-lived and abnormal proteins. Down-regulated during the S-phase it is involved in progression through the cell cycle. Regulates nuclear hormone receptors transcriptional activity. May play a role in myelopoiesis.
Short Description: Recombinant UBE2L3 protein that includes full length of human UBE2L3 protein (accession number NP_003338.1) was expressed in E. coli and contains an N-terminal His tag with a molecular weight of 20.02 kDa. The purity of the protein is ≥ 95 % by SDS-PAGE.
Aliases: Ubiqutin conjugating enzyme E2 L3, E2-F1, L-UBC, UBCH7, UbcM4, UBCE7
Target
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