UBE2M Protein

Catalog No. ABIN7939710

This Recombinant UBE2M protein is expressed in Escherichia coli (E. coli).

Quick Overview for UBE2M Protein (ABIN7939710)

Target: UBE2M (ube2m) (Ubiquitin-Conjugating Enzyme E2M (ube2m))

Protein Type: Recombinant

Origin: Human

Source: Escherichia coli (E. coli)

Application: Activity Assay (AcA), Functional Studies (Func), Screening Assay (ScA)

Product Details

Purpose: Recombinant UBE2M protein

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Recombinant UBE2M protein is supplied in 25 mM Tris 8.0, 300 mM NaCl, 20 % glycerol, 0.5 mM TCEP.

Handling Advice: Avoid repeated freeze/thaw cycles,keep on ice when not in storage

Storage: -80 °C

Storage Comment: Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

Target Details

Target: UBE2M (ube2m) (Ubiquitin-Conjugating Enzyme E2M (ube2m))

Alternative Name: UBE2M

Background:

Protein Background: UBE2M (NEDD8-conjugating enzyme Ubc12) also known as hUbc12, UBC-RS2 and UBC12 UBE2M can accepts the ubiquitin-like protein NEDD8 from the UBA3-NAE1 E1 complex and catalyzes its covalent attachment to other proteins. The specific interaction with the E3 ubiquitin ligase RBX1, but not RBX2, suggests that the RBX1-UBE2M complex neddylates specific target proteins, such as CUL1, CUL2, CUL3 and CUL4. Involved in cell proliferation.

Short Description: Recombinant UBE2M protein that includes full length of human UBE2M protein (accession number NP_003960.1) was expressed in E. coli and contains an N-terminal His tag with a molecular weight of 23.06 kDa. The purity of the protein is ≥ 95 % by SDS-PAGE.

Aliases: NEDD8-conjugating enzyme Ubc12, hUbc12, UBC-RS2, NEDD8-Carrier Protein, EC 2.3.2.34