YTHDC1 Protein (full length)

Catalog No. ABIN7939762

Recombinant YTHDC1 protein expressed in Baculovirus infected Insect Cells.

Quick Overview for YTHDC1 Protein (full length) (ABIN7939762)

Target: YTHDC1 (YTH Domain Containing 1 (YTHDC1))

Protein Type: Recombinant

Origin: Human

Source: Baculovirus infected Insect Cells

Application: Screening Assay (ScA), Activity Assay (AcA), Functional Studies (Func)

Product Details

Protein Characteristics: full length

Purpose: Recombinant YTHDC1 protein

Characteristics: Recombinant YTHDC1 protein was expressed in a baculovirus expression system as the full-length protein (accession number NP_001026902.1) with an N-terminal Flag tag. The molecular weight of the protein is 85.6 kDa. It is suitable for use in binding assays, inhibitor screening, and selectivity profiling.

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Recombinant YTHDC1 protein is supplied in 25 mM HEPES-NaOH pH 7.5, 300 mM NaCl, 10 % glycerol, 0.04 % Triton X-100, 0.5 mM TCEP.

Handling Advice: Avoid repeated freeze/thaw cycles,keep on ice when not in storage

Storage: -80 °C

Storage Comment: Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

Target Details

Target: YTHDC1 (YTH Domain Containing 1 (YTHDC1))

Alternative Name: YTHDC1

Background:

Protein Background: YTHDC1 (YTH Domain Containing 1), is a regulator of alternative splicing that specifically recognizes and binds N6-methyladenosine (m6A)-containing RNAs. M6A is a modification present at internal sites of mRNAs and some non-coding RNAs and plays a role in the efficiency of mRNA splicing, processing and stability. YTHDC1 acts as a key regulator of exon-inclusion or exon-skipping during alternative splicing via interaction with mRNA splicing factors SRSF3 and SRSF10. It specifically binds m6A-containing mRNAs and promotes recruitment of SRSF3 to its mRNA-binding elements adjacent to m6A sites, leading to exon-inclusion during alternative splicing. In contrast, interaction with SRSF3 prevents interaction with SRSF10, a splicing factor that promotes exon skipping: this prevents SRSF10 from binding to its mRNA-binding sites close to m6A-containing regions, leading to inhibit exon skipping during alternative splicing. YTHDC1 may also regulate alternative splice site selection.

Short Description: Recombinant YTHDC1 protein was expressed in a baculovirus expression system as the full length protein (accession number NP_001026902.1) with an N-terminal FLAG tag. The molecular weight of the protein is 85.6 kDa.

Aliases: YTH Domain Containing 1, Splicing Factor YT521

Molecular Weight: 85.6 kDa

NCBI Accession: NP_001026902