ADPRHL2 Protein

Catalog No. ABIN7939788

This Recombinant ADPRHL2 protein is expressed in Escherichia coli (E. coli).

Quick Overview for ADPRHL2 Protein (ABIN7939788)

Target: ADPRHL2 (ADP-Ribosylhydrolase Like 2 (ADPRHL2))

Protein Type: Recombinant

Origin: Human

Source: Escherichia coli (E. coli)

Application: Functional Studies (Func), Screening Assay (ScA), Activity Assay (AcA)

Product Details

Purpose: Recombinant ADPRS / ARH3 protein

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Recombinant ADPRS / ARH3 protein is supplied in 25 mM Tris 8.0, 300 mM NaCl, 20 % glycerol, 0.5 mM TCEP.

Handling Advice: Avoid repeated freeze/thaw cycles,keep on ice when not in storage

Storage: -80 °C

Storage Comment: Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

Target Details

Target: ADPRHL2 (ADP-Ribosylhydrolase Like 2 (ADPRHL2))

Alternative Name: ADPRS & ARH3

Background:

Protein Background: Protein ADP-ribosylation is a highly dynamic post-translational modification. The rapid turnover is achieved, among others, by ADP (ribosyl)hydrolases (ARHs), an ancient family of enzymes that reverses this modification. Recently ARHs came into focus due to their role as regulators of cellular stresses and tumor suppressors. ADPRS / ARH3 (ADPribosylhydrolase ARH3) as one of ARHs, it plays a variety of functions in organisms.It preferentially hydrolyzes the scissile alpha-O-linkage attached to the anomeric C1'' position of ADP-ribose and acts on different substrates, such as proteins ADP-ribosylated on serine and threonine, free poly(ADP-ribose) and O-acetyl-ADP-D ribose. Specifically acts as a serine mono-ADP-ribosylhydrolase by mediating the removal of mono-ADP-ribose attached to serine residues on proteins, thereby playing a key role in DNA damage response. Also able to degrade protein free poly(ADP-ribose), which is synthesized in response to DNA damage: free poly(ADP-ribose) acts as a potent cell death signal and its degradation by ADPRHL2 protects cells from poly(ADP-ribose)-dependent cell death, a process named parthanatos.

Short Description: Recombinant ADPRS / ARH3 protein that includes full length of the human ADPRS / ARH3 protein (accession number NP_060295.1) was expressed in E.coli cells with an N-terminal His tag, The molecular weight of the protein is 41.11 kDa.

Aliases: ADP-Ribosylserine Hydrolase, ARH3, ADPRHL2, ADPRS, ADPRHL2, FLJ20446