TET1 Protein (AA 1634-1986, AA 2601-2708)

Catalog No. ABIN7939796

This Recombinant TET1 protein is produced in Escherichia coli (E. coli).

Quick Overview for TET1 Protein (AA 1634-1986, AA 2601-2708) (ABIN7939796)

Target: TET1 (Tet Methylcytosine Dioxygenase 1 (TET1))

Protein Type: Recombinant

Origin: Drosophila melanogaster

Source: Escherichia coli (E. coli)

Application: Activity Assay (AcA), Functional Studies (Func), Screening Assay (ScA)

Product Details

Protein Characteristics: AA 1634-1986, AA 2601-2708

Purpose: Recombinant DmTet (1634-1986, 2601-2708) protein

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Recombinant DmTet (1634-1986 and 2601-2708) protein is supplied in 25 mM Tris pH 7.4, 300 mM NaCl, 10 % glycerol, and 0.5 mM TCEP.

Handling Advice: Avoid repeated freeze/thaw cycles,keep on ice when not in storage

Storage: -80 °C

Storage Comment: Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

Target Details

Target: TET1 (Tet Methylcytosine Dioxygenase 1 (TET1))

Alternative Name: DmTet

Background:

Protein Background: Ten Eleven Translocation (TET) family proteins are cytosine oxygenases that catalyze the conversion of 5-methylcytosine (5mC) into 5-hydroxymethylcytosine (5hmC). 5hmC can be further oxidized into 5-formylcytosine (5fC) and 5-carboxylcytosine (5caC) by TET proteins. Methylation at the C5 position of cytosine is an epigenetic modification of the mammalian genome that plays an important role in transcriptional regulation. TET proteins are responsible for initiating the enzymatic deamination process leading to cytosine demethylation.

Short Description: Recombinant DmTet (1634-1986 and 2601-2708) protein was expressed in E. colicells as the Protein fragment (accession number NP_001246581.1) with an N-terminal 6xHis tag. The molecular weight of DmTet (1634-1986 and 2601-2708) is 53.68 kDa.

Aliases: Drosophila melanogaster Tet-like protein

Pathways: Stem Cell Maintenance, Warburg Effect