PI3K p85/p55 Protein (Complex)

Catalog No. ABIN7939821

This Recombinant PI3K p85/p55 protein is produced in Baculovirus infected Insect Cells.

Quick Overview for PI3K p85/p55 Protein (Complex) (ABIN7939821)

Target: PI3K p85/p55 (Phosphoinositide 3 Kinase, p85/p55 (PI3K p85/p55))

Protein Type: Recombinant

Origin: Human

Source: Baculovirus infected Insect Cells

Application: Activity Assay (AcA), Functional Studies (Func), Screening Assay (ScA)

Product Details

Protein Characteristics: Complex

Purpose: Recombinant PI3Ka / p85 protein complex

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Recombinant PI3Ka / p85 Complex is supplied in 25 mM HEPES pH 7.5, 300 mM NaCl, 20 % glycerol, 0.04 % Triton X-100, 0.5 mM TCEP.

Handling Advice: Avoid repeated freeze/thaw cycles,keep on ice when not in storage

Storage: -80 °C

Storage Comment: Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

Target Details

Target: PI3K p85/p55 (Phosphoinositide 3 Kinase, p85/p55 (PI3K p85/p55))

Alternative Name: PI3Ka & p85

Background:

Protein Background: Phosphoinositide 3-kinase (PI3K) family of lipid kinases are divided into three major classes based on primary sequence, substrate preference, and regulation. While class IA PI3Ks are heterodimeric enzymes composed of a catalytic subunit (p110a, p110b, or p110d) complexed with one of five regulatory subunits (p85a, p55a, p50a, p85b, or p55g), the classIB enzyme is a dimer made of p110g catalytic subunit and p101or p84 regulatory subunit. The class I catalytic subunit polypeptides p110a, p110b, p110d, and p110g are encoded by PIK3CA, PIK3CB, PIK3CD, and PIK3CG, respectively. The regulatory subunits are encoded by five genes: PIK3R1 codes p85a, p55a, and p50a, PIK3R2 codes p85b, PIK3R3 codes p55g, PIK3R5 codes p101, and PIK3R6 codes p84. PI3K is a lipid and protein kinase that can be activated by receptor tyrosine kinases, cytokine receptors, chemokine G protein-coupled receptors, or oncogenes, including RAS. PI3K converts phosphatidylinositol 4,5-bisphosphate (PIP2) to phosphatidylinositol-3,4,5-triphosphate (PIP3), which leads to activation of pyruvate dehydrogenase kinase 1 (PDK1) and the serine/threonine kinase AKT (PKB) (7). AKT has many downstream effectors, which regulate the cell cycle, apoptosis, protein translation, autophagy, and metabolism. Most cytokines and RTK ligands signal through class IA PI3K.

Short Description: Recombinant PI3Ka / p85 Complex that includes full length human PI3Ka protein (accession number NP_006209.2) with an N-terminal FLAG tag and full length human p85 protein (accession number NP_852664.1) without tag was expressed in Hi5 cells. The molecular weights of PI3Ka and p85 are 125.6 kDa and 83.6 kDa, respectively.

Aliases: Phosphoinositide 3-kinase, PI3K, P110-Alpha, CLAPO, CLOVE, CCM4, MCAP, MCM