SARS-CoV-2 NSP12 (RdRP) Protein
Quick Overview for SARS-CoV-2 NSP12 (RdRP) Protein (ABIN7939833)
Target
Protein Type
Origin
Source
Application
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Purpose
- Recombinant SARS-CoV-2 NSP12 / RdRp protein
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Application Notes
- Optimal working dilution should be determined by the investigator.
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Restrictions
- For Research Use only
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Format
- Liquid
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Buffer
- Recombinant SARS-CoV-2 NSP12 / RdRp protein is supplied in 25 mM HEPES-NaOH pH 7.5, 300 mM NaCl, 10 % glycerol, 0.04 % Triton X-100, and 0.5 mM TCEP.
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Handling Advice
- Avoid repeated freeze/thaw cycles,keep on ice when not in storage
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Storage
- -80 °C
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Storage Comment
- Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.
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- SARS-CoV-2 NSP12 (RdRP) (SARS-CoV-2 RdRP) (SARS-CoV-2 RNA-dependent RNA Polymerase (NSP12) (SARS-CoV-2 RdRP))
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Alternative Name
- SARS-CoV-2 NSP12 & RdRp
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Background
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Protein Background: Coronavirus replication and transcription machinery involves multiple virus-encoded nonstructural proteins (nsp). Coronaviruses are enveloped positive-stranded RNA viruses with the largest currently known RNA genomes. Expression of their genomes begins with the translation of two large replicase polyproteins, pp1a (>4,000 residues) and pp1ab (>7,000 residues), which are encoded by the viral replicase gene that comprises open reading frame 1a (orf1a) and orf1b1. pp1a and pp1ab are extensively processed by orf1a-encoded proteases to yield 15 or 16 mature nonstructural (replicase) proteins that assemble to form the membrane-associated viral replication and transcription machinery, which is vital to the viral life cycle. The nsp12, which is the first protein encoded by ORF1b, has common viral RNA-dependent RNA polymerase (RdRp) motifs and is thus the catalytic core of the RNA replicase.
Short Description: Recombinant SARS-CoV-2 NSP12 / RdRp protein was expressed in baculovirus expression system as the full length protein (accession number YP_009724389.1) with a N-terminal FLAG tag. The molecular weight of the protein is 107.9 kDa.
Aliases: Nonstructural Protein 12
Target
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