LGMN Protein
Quick Overview for LGMN Protein (ABIN7964652)
Target
See all LGMN ProteinsProtein Type
Origin
Source
Purity
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Purpose
- Legumain, Asparaginyl Endopeptidase (AEP), human, recombinant, Leishmania
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Characteristics
- Legumain is a cysteine protease with a strict specificity for cleaving after asparagine and, to a lesser extent, aspartic acid residues. Therefore, it is synonymously named the asparaginyl endopeptidase or AEP. Its very strict substrate specificity makes it an ideal enzyme for example for digestion proteomics. Human legumain is synthesized as an inactive proenzyme (56 kDa) composed of the caspase-like catalytic AEP domain (36 kDa) and a C-terminal death-domain-like prodomain. Activation to the active AEP proceeds via the pH -dependent autocatalytic release of the C-terminal prodomain at acidic pH . While prolegumain is stable at neutral pH , AEP is stable at acidic pH (≤ 6.0) but will irreversibly unfold at near neutral pH conditions. The pH -optimum of its endopeptidase activity is at pH 5.5 with a strong preference for cleaving after asparagine residues. Hydrolysis of aspartyl peptide bonds is preferentially catalyzed at more acidic pH ( pH 4.0). In addition to its well established protease activity, legumain harbors a pH -dependent ligase activity at near neutral pH .
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Legumain (LGMN) (AA 1-433) protein (GST tag)
LGMN Origin: Human Host: Wheat germ Recombinant WB, ELISA, AP, AA
Legumain (LGMN) (Active) protein (His tag)LGMN Origin: Mouse Host: HEK-293 Cells Recombinant > 75 % as determined by SDS-PAGE Active
Legumain (LGMN) protein (His tag)LGMN Origin: Mouse Host: Human Cells Recombinant > 95 % as determined by SDS-PAGE
Legumain (LGMN) (AA 18-433) protein (His-IF2DI Tag)LGMN Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 90 % as determined by SDS-PAGE. WB, ELISA
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Comment
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Optional[Concentration Comments]: 500 μg/mL
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Restrictions
- For Research Use only
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Format
- Liquid
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Buffer
- supplied in 20 mM citric acid pH 4.0100 mM NaCl2 mM DTT
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Preservative
- Dithiothreitol (DTT)
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Precaution of Use
- This product contains Dithiothreitol (DTT): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Handling Advice
- Please centrifuge briefly before opening (volume ≤2 ml).,avoid freeze/thaw cycles
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Storage
- -20 °C
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Storage Comment
- store at -20 °C avoid freeze/thaw cycles
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Expiry Date
- 12 months
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- LGMN (Legumain (LGMN))
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Alternative Name
- Legumain
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Molecular Weight
- 36 kDa
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UniProt
- Q99538
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Pathways
- Metabolism of Steroid Hormones and Vitamin D, Activation of Innate immune Response, Toll-Like Receptors Cascades
Target
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