IFN alpha 2b Protein (AA 24-188)

Catalog No. ABIN8056968

This Recombinant IFN alpha 2b protein is produced in Escherichia coli (E. coli).

Quick Overview for IFN alpha 2b Protein (AA 24-188) (ABIN8056968)

Target: IFN alpha 2b

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: Escherichia coli (E. coli)

Application: Cell Culture (CC)

Purity: > 95 % as determined by reducing SDS-PAGE.

Product Details

Protein Characteristics: AA 24-188

Purpose: Recombinant Human IFNα2b/IFNA2 Protein

Sequence: Cys24-Glu188

Sterility: 0.2 μm filtered

Endotoxin Level: < 1.0 EU per μg of the protein as determined by the LAL method.

Biological Activity Comment: Measured in antiviral assay using A549 human lung cancer cells infected with vesicular stomatitisvirus (VSV) The ED₅₀ for this effect is 5 ng/mL.

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Buffer: Lyophilized from a 0.2 μm filtered solution of 20 mM PB, 150 mM NaCl, pH 7.2.
Normally 5 % - 8 % trehalose, mannitol and 0.01 % Tween 80 are added as protectants before lyophilization.

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Expiry Date: 12 months

Target Details

Target: IFN alpha 2b

Alternative Name: IFNalpha2b

Background: IFN-Alpha-2,IFN-alpha 2b,IFNA2,Interferon Alpha-2,Interferon Alpha-A,LeIF A,At least 23 different variants of IFN-α are known. The individual proteins have molecular masses between 19-26 kDa and consist of proteins with lengths of 156-166 and 172 amino acids. All IFN-α subtypes possess a common conserved sequence region between amino acid positions 115-151 while the amino-terminal ends are variable. Many IFN-α subtypes differ in their sequences by only one or two positions. Naturally occurring variants also include proteins that are truncated by 10 amino acids at the carboxyl-terminal end.

Molecular Weight:

Calculated MW: 19.4 kDa

Observed MW: 17 kDa

Gene ID: 3440

UniProt: P01563