Lipocalin 2 Protein (LCN2) (AA 21-200)

Catalog No. ABIN8057031

This Recombinant Lipocalin 2 protein is produced in Mammalian Cells.

Quick Overview for Lipocalin 2 Protein (LCN2) (AA 21-200) (ABIN8057031)

Target: Lipocalin 2 (LCN2)

Protein Type: Recombinant

Origin: Mouse

Source: Mammalian Cells

Application: Immunogen (Imm)

Purity: > 95 % as determined by reducing SDS-PAGE.

Product Details

Protein Characteristics: AA 21-200

Purpose: Recombinant Mouse Lcn2 Protein(Halo Tag)

Sequence: Gln21-Asn200

Sterility: 0.2 μm filtered

Endotoxin Level: < 1.0 EU/mg of the protein as determined by the LAL method

Biological Activity Comment: Not validated for activity

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.5 mg/mL. Concentration is measured by UV-Vis.

Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with 5 % Trehalose and 5 % Mannitol.

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Expiry Date: 12 months

Target Details

Target: Lipocalin 2 (LCN2)

Alternative Name: Lcn2

Background: Lipocalin-2,NGAL,24p3,Lipocalin-2 (LCN2), also known as neutrophil gelatinase-associated lipocalin (NGAL), is a 25 kDa protein belonging to the lipocalin superfamily. It was initially found in activated neutrophils, however, many other cells, like kidney tubular cells, may produce NGAL in response to various insults. This protein is released from injured tubular cells after various damaging stimuli, is already known by nephrologists as one of the most promising biomarkers of incoming Acute Kidney Injury (AKI). Recent evidence also suggests its role as a biomarker in a variety of other renal and non-renal conditions. Moreover, recent studies seem to suggest a potential involvement of this factor also in the genesis and progression of chronic kidney diseases. NGAL is the first known mammalian protein which specifically binds organic molecules called siderophores, which are high-affinity iron chelators. NGAL, first known as an antibacterial factor of natural immunity, and an acute phase protein, is currently one of the most interesting and enigmatic proteins involved in the process of tumor development. acting as an intracellular iron carrier and protecting MMP9 from proteolytic degradation, NGAL has a clear pro-tumoral effect. In thyroid carcinomas, NGAL is strongly induced by NF-kB, an important factor involved both in tumor growth and in the link between chronic inflammation and neoplastic development. Thus, Lipocalin-2 (LCN2/NGAL) has been implicated in a variety of processes including cell differentiation, proliferation, survival and morphogenesis.

Molecular Weight:

Calculated MW: 53 kDa

Observed MW: 53 kDa

UniProt: P11672

Pathways: Cellular Response to Molecule of Bacterial Origin, Transition Metal Ion Homeostasis