DNMT3B Protein (AA 145-355)

Catalog No. ABIN8127613

This Recombinant DNMT3B protein is expressed in HEK-293F Cells.

Quick Overview for DNMT3B Protein (AA 145-355) (ABIN8127613)

Target: DNMT3B (DNA (Cytosine-5-)-Methyltransferase 3 beta (DNMT3B))

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: HEK-293F Cells

Application: Activity Assay (AcA), Cell Culture (CC)

Purity: > 95 %

Product Details

Protein Characteristics: AA 145-355

Purpose: Active DNA Methyltransferase 3B (DNMT3B)

Sequence: Ser145-Pro355

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

STABILITY: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Isoelectric Point: 7

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Reconstitute in 20 mM Tris, 150 mM NaCl ( pH 8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

Buffer: PBS, pH 7.4, containing 0.01 % SKL, 1 mM DTT, 5 % Trehalose and Proclin300.

Preservative: Dithiothreitol (DTT), ProClin

Precaution of Use: This product contains ProClin and Dithiothreitol (DTT): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Storage: 4 °C,-80 °C

Storage Comment: Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.

Target Details

Target: DNMT3B (DNA (Cytosine-5-)-Methyltransferase 3 beta (DNMT3B))

Alternative Name: DNA Methyltransferase 3B

Background: ICF, M.HsaIIIB, DNA methyltransferase HsaIIIB

Molecular Weight: 30 & 33kDa

UniProt: Q9UBC3