NOS2 Protein (AA 43-213)
Quick Overview for NOS2 Protein (AA 43-213) (ABIN8128857)
Target
See all NOS2 ProteinsProtein Type
Biological Activity
Origin
Source
Application
Purity
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Protein Characteristics
- AA 43-213
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Purpose
- Active Nitric Oxide Synthase 2, Inducible (NOS2)
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Sequence
- Asp43-Thr213
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Nitric Oxide Synthase 2, Inducible (NOS2) (AA 1-1153) protein (His tag)
custom-made NOS2 Origin: Human Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
Nitric Oxide Synthase 2, Inducible (NOS2) (AA 19-221) protein (His tag)NOS2 Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 90 % as determined by SDS-PAGE. WB, ELISA
Nitric Oxide Synthase 2, Inducible (NOS2) (AA 1-196) protein (His tag)NOS2 Origin: Rat Host: Escherichia coli (E. coli) Recombinant Greater than 90 % as determined by SDS-PAGE. WB, ELISA
Nitric Oxide Synthase 2, Inducible (NOS2) (AA 685-794) protein (GST tag)NOS2 Origin: Human Host: Wheat germ Recombinant AP, AA, ELISA, WB
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Application Notes
- Optimal working dilution should be determined by the investigator.
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Comment
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STABILITY: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
Isoelectric Point: 7.8
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Restrictions
- For Research Use only
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Format
- Lyophilized
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Reconstitution
- Reconstitute in 10 mM PBS ( pH 7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
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Buffer
- 20 mM Tris, 150 mM NaCl, pH 8.0, containing 1 mM EDTA, 1 mM DTT, 0.01 % SKL, 5 % Trehalose and Proclin300.
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Preservative
- Dithiothreitol (DTT), Other preservative, ProClin
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Precaution of Use
- This product contains ProClin and Dithiothreitol (DTT): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.
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Storage
- 4 °C,-80 °C
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Storage Comment
- Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
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- NOS2 (Nitric Oxide Synthase 2, Inducible (NOS2))
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Alternative Name
- Nitric Oxide Synthase 2, Inducible
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Background
- NOS2A, INOS, HEP-NOS, I-NOS, Hepatocytes Oxide Synthase, Peptidyl-cysteine S-nitrosylase NOS2
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Molecular Weight
- 20kDa
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UniProt
- P29477
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Pathways
- Retinoic Acid Receptor Signaling Pathway, Cellular Response to Molecule of Bacterial Origin, Inositol Metabolic Process, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process
Target
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