PAM Protein (AA 21-288)
Quick Overview for PAM Protein (AA 21-288) (ABIN8128932)
Target
See all PAM ProteinsProtein Type
Biological Activity
Origin
Source
Application
Purity
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Protein Characteristics
- AA 21-288
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Purpose
- Active Peptidylglycine Alpha Amidating Monooxygenase (PAM)
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Sequence
- Phe21-Cys288
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Peptidylglycine alpha-Amidating Monooxygenase (PAM) (AA 1-866) protein (GST tag)
PAM Origin: Human Host: Wheat germ Recombinant AP, AA, ELISA, WB
Peptidylglycine alpha-Amidating Monooxygenase (PAM) (Transcript Variant 3) (Active) protein (Myc-DYKDDDDK Tag)PAM Origin: Human Host: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, Func, PI, STD Active
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Application Notes
- Optimal working dilution should be determined by the investigator.
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Comment
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STABILITY: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
Isoelectric Point: 8.8
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Restrictions
- For Research Use only
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Format
- Lyophilized
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Reconstitution
- Reconstitute in 20 mM Tris, 150 mM NaCl (PH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
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Buffer
- 20 mM Tris, 150 mM NaCl, pH 8.0, containing 1 mM EDTA, 1 mM DTT, 0.01 % SKL, 5 % Trehalose and Proclin300.
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Preservative
- Dithiothreitol (DTT), Other preservative, ProClin
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Precaution of Use
- This product contains ProClin and Dithiothreitol (DTT): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.
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Storage
- 4 °C,-80 °C
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Storage Comment
- Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
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- PAM (Peptidylglycine alpha-Amidating Monooxygenase (PAM))
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Alternative Name
- Peptidylglycine Alpha Amidating Monooxygenase
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Background
- PAL, PHM, Peptidylamidoglycolate lyase, Peptidyl-Alpha-hydroxyglycine Alpha-amidating Lyase, Peptidylglycine Alpha-Hydroxylating Monooxygenase
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Molecular Weight
- 34kDa
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UniProt
- P19021
Target
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