PKM2 Protein (AA 294-470)

Catalog No. ABIN8129123

Recombinant PKM2 protein expressed in Escherichia coli (E. coli).

Quick Overview for PKM2 Protein (AA 294-470) (ABIN8129123)

Target: PKM2 (Pyruvate Kinase M2 (PKM2))

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: Escherichia coli (E. coli)

Application: Activity Assay (AcA), Cell Culture (CC)

Purity: > 97 %

Product Details

Protein Characteristics: AA 294-470

Purpose: Active Pyruvate kinase isozymes M2 (PKM2)

Sequence: Arg294-Phe470

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

STABILITY: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Isoelectric Point: 8.3

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Reconstitute in 10 mM PBS ( pH 7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

Buffer: 20 mM Tris, 150 mM NaCl, pH 8.0, containing 1 mM EDTA, 1 mM DTT, 0.01 % SKL, 5 % Trehalose and Proclin300.

Preservative: Dithiothreitol (DTT), Other preservative, ProClin

Precaution of Use: This product contains ProClin and Dithiothreitol (DTT): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Storage: 4 °C,-80 °C

Storage Comment: Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.

Target Details

Target: PKM2 (Pyruvate Kinase M2 (PKM2))

Alternative Name: Pyruvate kinase isozymes M2

Background: M2PK, PKM, M2-PK, PKM1, CTHBP, OIP3, PK3, PKM, TCB, THBP1, Pyruvate kinase muscle isozyme, Thyroid hormone-binding protein 1, Cytosolic thyroid hormone-binding protein, Pyruvate Kinase, Muscle

Molecular Weight: 50kDa

UniProt: P14618

Pathways: Warburg Effect