SIRT4 Protein (AA 28-314)

Catalog No. ABIN8129277

This Recombinant SIRT4 protein is produced in Escherichia coli (E. coli).

Quick Overview for SIRT4 Protein (AA 28-314) (ABIN8129277)

Target: SIRT4 (Sirtuin 4 (SIRT4))

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: Escherichia coli (E. coli)

Application: Activity Assay (AcA), Cell Culture (CC)

Purity: > 97 %

Product Details

Protein Characteristics: AA 28-314

Purpose: Active Sirtuin 4 (SIRT4)

Sequence: Ser28-Cys314

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

STABILITY: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Isoelectric Point: 8.8

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Reconstitute in 20 mM Tris, 150 mM NaCl ( pH 8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

Buffer: 20 mM Tris, 150 mM NaCl, pH 8.0, containing 1 mM EDTA, 1 mM DTT, 0.01 % SKL, 5 % Trehalose and Proclin300.

Preservative: Dithiothreitol (DTT), Other preservative, ProClin

Precaution of Use: This product contains ProClin and Dithiothreitol (DTT): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Storage: 4 °C,-80 °C

Storage Comment: Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.

Target Details

Target: SIRT4 (Sirtuin 4 (SIRT4))

Alternative Name: Sirtuin 4

Background: SIR2L4, Silent Mating Type Information Regulation 2 Homolog 4, SIR2-like protein 4, NAD-dependent protein deacetylase sirtuin-4

Molecular Weight: 36kDa

UniProt: Q9Y6E7

Pathways: Negative Regulation of Hormone Secretion