SMPD1 Protein (AA 58-626)
Quick Overview for SMPD1 Protein (AA 58-626) (ABIN8129308)
Target
See all SMPD1 ProteinsProtein Type
Biological Activity
Origin
Source
Application
Purity
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Protein Characteristics
- AA 58-626
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Purpose
- Active Acid Sphingomyelinase (ASM)
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Sequence
- Tyr58-Leu626
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Sphingomyelin phosphodiesterase 1, Acid Lysosomal (SMPD1) (Active) protein (His tag)
SMPD1 Origin: Human Host: Baculovirus infected Insect Cells Recombinant > 90 % as determined by reducing SDS-PAGE. Active
Sphingomyelin phosphodiesterase 1, Acid Lysosomal (SMPD1) (Active) protein (His tag)SMPD1 Origin: Mouse Host: Baculovirus infected Insect Cells Recombinant > 85 % as determined by reducing SDS-PAGE. Active
Sphingomyelin phosphodiesterase 1, Acid Lysosomal (SMPD1) (AA 531-630) protein (GST tag)SMPD1 Origin: Human Host: Wheat germ Recombinant AP, AA, ELISA, WB
Sphingomyelin phosphodiesterase 1, Acid Lysosomal (SMPD1) (AA 1-364) protein (GST tag)SMPD1 Origin: Human Host: Wheat germ Recombinant AP, AA, ELISA, WB
Sphingomyelin phosphodiesterase 1, Acid Lysosomal (SMPD1) (Transcript Variant 1) protein (Myc-DYKDDDDK Tag)SMPD1 Origin: Human Host: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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Application Notes
- Optimal working dilution should be determined by the investigator.
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Comment
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STABILITY: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
Isoelectric Point: 6.9
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Restrictions
- For Research Use only
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Format
- Lyophilized
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Reconstitution
- Reconstitute in 10 mM PBS ( pH 7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
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Buffer
- PBS, pH 7.4, containing 0.01 % SKL, 1 mM DTT, 5 % Trehalose and Proclin300.
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Preservative
- Dithiothreitol (DTT), ProClin
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Precaution of Use
- This product contains ProClin and Dithiothreitol (DTT): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.
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Storage
- 4 °C,-80 °C
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Storage Comment
- Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
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- SMPD1 (Sphingomyelin phosphodiesterase 1, Acid Lysosomal (SMPD1))
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Alternative Name
- Acid Sphingomyelinase
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Background
- SMPD1, NPD, SMase, aSMase, Sphingomyelin Phosphodiesterase 1,Acid Lysosomal, Simply Sphingomyelinase
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Molecular Weight
- 75kDa
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UniProt
- Q04519
Target
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