Streptavidin Protein (AA 25-183) (His tag)

Catalog No. ABIN8129322

This Recombinant Streptavidin protein is produced in HEK-293F Cells.

Quick Overview for Streptavidin Protein (AA 25-183) (His tag) (ABIN8129322)

Target: Streptavidin

Protein Type: Recombinant

Origin: Various Species

Source: HEK-293F Cells

Application: Western Blotting (WB), SDS-PAGE (SDS), Positive Control (PC), Immunogen (Imm)

Purity: > 95 %

Product Details

Protein Characteristics: AA 25-183

Purification tag / Conjugate: This Streptavidin protein is labelled with His tag.

Purpose: Eukaryotic Streptavidin (SA)

Sequence: Asp25-Gln183

Endotoxin Level: <1.0EU per 1μg (determined by the LAL method)

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

STABILITY: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Isoelectric Point: 6.5

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Reconstitute in 20 mM Tris, 150 mM NaCl ( pH 8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

Buffer: PBS, pH 7.4, containing 0.01 % SKL, 1 mM DTT, 5 % Trehalose and Proclin300.

Preservative: Dithiothreitol (DTT), ProClin

Precaution of Use: This product contains ProClin and Dithiothreitol (DTT): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Storage: 4 °C,-80 °C

Storage Comment: Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.

Target Details

Target: Streptavidin

Molecular Weight: 20 & 80kDa

UniProt: P22629