FASL Protein (AA 103-281) (DYKDDDDK Tag)
Quick Overview for FASL Protein (AA 103-281) (DYKDDDDK Tag) (ABIN925124)
Target
See all FASL ProteinsProtein Type
Origin
Source
Application
Purity
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Protein Characteristics
- AA 103-281
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Purification tag / Conjugate
- This FASL protein is labelled with DYKDDDDK Tag.
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Specificity
- RhsFasL binds to human, mouse and rat Fas (CD95, APO-1).
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Characteristics
- Results using sFasL may differ from those obtained with agonistic antibodies. Host: The extracellular domain of human FasL (sFasL, aa. 103-281) is fused at the N-terminus to a linker peptide (26 AA) and a DYKDDDDK Tag. Glycosylation of rhsFasL is similar to natural human FasL.
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Endotoxin Level
- <0.1 EU/µg purified protein as determined by LAL test.
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Fas Ligand (TNF Superfamily, Member 6) (FASL) (AA 134-281) (Active) protein (His tag)
FASL Origin: Human Host: HEK-293 Cells Recombinant > 95 % as determined by Bis-Tris PAGE Active
Fas Ligand (TNF Superfamily, Member 6) (FASL) (AA 134-281) (Active) protein (His tag)FASL Origin: Human Host: HEK-293 Cells Recombinant >90 % as determined by SDS-PAGE. Active
Fas Ligand (TNF Superfamily, Member 6) (FASL) (AA 99-330) protein (Fc Tag)FASL Origin: Human Host: HEK-293 Cells Recombinant The purity of the protein is greater than 95 % as determined by SDS-PAGE and Coomassie blue staining. ScA, IA, ADA, AM
Fas Ligand (TNF Superfamily, Member 6) (FASL) proteinFASL Origin: Human Host: Escherichia coli (E. coli) Recombinant > 90 % pure SDS
Fas Ligand (TNF Superfamily, Member 6) (FASL) (AA 1-281) protein (GST tag)FASL Origin: Human Host: Wheat germ Recombinant AP, AA, ELISA, WB
Fas Ligand (TNF Superfamily, Member 6) (FASL) (AA 134-281) (Active) protein (His tag)FASL Origin: Human Host: HEK-293 Cells Recombinant > 95 % as determined by reducing SDS-PAGE. BLI Active
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Application Notes
- Induction of Apoptosis: Recombinant FasL protein induces apoptosis in Fas-sensitive cells (ED50: 50 ng/mL in A20 cells). Optimal concentration varies with cell type and should be determined by testing serial dilutions on cells. Alone, rhsFasL kills Fas-sensitive cells at a concentration of >10 ng/mL. Use of a secondary enhancing cross-linking antibody reacting with rhsFasL (Cat. No. FL-104) increases the activity of rhsFasL approximately 50-fold.
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Restrictions
- For Research Use only
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Format
- Lyophilized
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Reconstitution
- Soluble. Prepare sterile stock solution by dissolving rhsFasL in 100 µL sterile water (0.1 mg/mL in PBS). Further dilutions should be made with medium containing 5% fetal calf serum.
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Buffer
- PBS
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Storage
- -20 °C
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- FASL (Fas Ligand (TNF Superfamily, Member 6) (FASL))
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Alternative Name
- Fas Ligand Protein
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Background
- Synonyms: APO-1L, soluble, CD95L, soluble, CD178, soluble, TNFSF 6, soluble Protein: Recombinant, human sFasL (rhsFasL)
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Molecular Weight
- ~32 kDa (non-glycosylated), ~35 kDa (glyco- sylated) (SDS-PAGE)
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Pathways
- Apoptosis, EGFR Signaling Pathway, Production of Molecular Mediator of Immune Response, Positive Regulation of Endopeptidase Activity
Target
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