HIV-1 Transmembrane Glycoprotein (HIV-1 gp41) (AA 546-682), (Ecto Domain) Protein

Catalog No. ABIN934613

Product Details

Target: HIV-1 Transmembrane Glycoprotein (HIV-1 gp41)

Protein Type: Recombinant

Protein Characteristics: Ecto Domain, AA 546-682

Origin: Human Immunodeficiency Virus (HIV)

Source: Yeast

Application: ELISA, Western Blotting (WB)

Characteristics: Purified recombinant HIV1 gp41 protein
Expression System: Yeast
This protein comprises of Ecto-domain of gp41 from Strain HxB2, amino acids 546 to 682

Purity: > 99 % pure

Application Details

Application Notes: Each Investigator should determine their own optimal working dilution for specific applications.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Supplied as a liquid in 20 mM Sodium Phosphate buffer, 500 mM NaCl, 0.2 % Triton X-100, and 6 M Urea, pH 7.4.

Handling Advice: Avoid repeated freeze/thaw cycles.

Storage: -80 °C

Storage Comment: Aliquot and store at -70 °C or lower.

References

Du, Idiart, Mariano, Chen, Jiang, Xu, Ostrow, Wrin, Phung, Binley, Petropoulos, Ballantyne, Whalen: "Effect of trimerization motifs on quaternary structure, antigenicity, and immunogenicity of a noncleavable HIV-1 gp140 envelope glycoprotein." in: Virology, Vol. 395, Issue 1, pp. 33-44, (2009) (PubMed).

Opalka, Pessi, Bianchi, Ciliberto, Schleif, McElhaugh, Danzeisen, Geleziunas, Miller, Eckert, Bramhill, Joyce, Cook, Magilton, Shiver, Emini, Esser: "Analysis of the HIV-1 gp41 specific immune response using a multiplexed antibody detection assay." in: Journal of immunological methods, Vol. 287, Issue 1-2, pp. 49-65, (2004) (PubMed).

Target Details

Target: HIV-1 Transmembrane Glycoprotein (HIV-1 gp41)

Alternative Name: HIV1 gp41

Target Type: Viral Protein

Background: HIV gp41 is a glycoprotein subunit that remains non-covalently-bound to gp120, and provides the second step by which HIV enters the cell. It is originally buried within the viral envelope, but, when gp120 binds to a CD4, gp120 changes its conformation, causing gp41 to become exposed, where it can assist in fusion with the host cell. The Env gene does not, in fact, code for gp120 and gp41, but for a precursor to both, gp160. During HIV reproduction, the host cell's own enzymes cleave gp160 into gp120 and gp41.
Alternative Names: HIV-1 gp41 protein, SU antigen, Surface protein antigen, HIV1 gp41, Envelope surface glycoprotein gp41 antigen, HIV-1 gp41, Human Immunodeficiency Virus 1 antigen, gp41 antigen, HIV 1 gp41, HIV 1 gp41 protein, gp41 glycoprotein antigen, gp41 antigen, Glycoprotein 41 antigen, HIV 1 gp41 antigen, HIV-1 gp41 antigen