GM-CSF Protein

Catalog No. ABIN934919

This Recombinant GM-CSF protein is produced in Escherichia coli (E. coli). There is 1 publication available.

Quick Overview for GM-CSF Protein (ABIN934919)

Target: GM-CSF (CSF2) (Colony Stimulating Factor 2 (Granulocyte-Macrophage) (CSF2))

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: Escherichia coli (E. coli)

Application: SDS-PAGE (SDS)

Purity: > 95 % pure

Product Details

Sequence: MAPARSPSPS TQPWEHVNAI QEARRLLNLS RDTAAEMNET VEVISEMFDL QEPTCLQTRL ELYKQGLRGS LTKLKGPLTM MASHYKQHCP PTPETSCATQ IITFESFKEN LKDFLLVIPF DCWEPVQE

Characteristics: Purified recombinant Human GMCSF protein
Expression System: E.coli
Bioactivity: The ED50 for this effect is < 0.18 ng/mL. Measured in a cell proliferation assay using TF1 human erythroleukemic cells.
Molecular weight on SDS-PAGE will appear higher.

Endotoxin Level: < 1.0 EU per µg of protein (determined by LAL method)

Application Details

Application Notes: GM-CSF protein has been used in SDS PAGE and may be suitable for use in other assays to be determined by the end user.

Assay Procedure:

Cell line: TF1 (human erythroleukemic cell).
Maintenance Condition: 10 % FBS RPMI 1640 with GM-CSF
Assay medium: 10 % FBS RPMI 1640 without Gm-CSF
Cell density: 2 x 10,000 cells/well (96 well plate, final volume 100 µL)
Starvation: 24 h,
Assay medium Incubation time: 24 hr (after sample treatment)
Concentration range: 0.048 ng/mL - 25 ng/mL
Detection method: MTT assay

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Supplied as a liquid in PBS, pH 7.4.

Handling Advice: Avoid repeated freeze/thaw cycles.

Storage: RT/-20 °C

Storage Comment: Store at 4 °C for short term storage (1/2 weeks). Aliquot and store at -20 °C or - 70 °C for long term storage.

References

Spatz, Eibl, Hink, Wolf, Fischer, Mayr, Schernthaner, Eibl: "Impaired primary immune response in type-1 diabetes. Functional impairment at the level of APCs and T-cells." in: Cellular immunology, Vol. 221, Issue 1, pp. 15-26, (2003) (PubMed).

Target Details

Target: GM-CSF (CSF2) (Colony Stimulating Factor 2 (Granulocyte-Macrophage) (CSF2))

Alternative Name: GMCSF

Background: Human GM-CSF is a 14.6 kDa globular protein consisting of 128 amino acid containing two disulfide bonds. GM-CSF is a hematopoietic growth factor that stimulates the development of neutrophils and macrophages and promotes the proliferation and development of early erythroid megakaryocytic and eosinophilic progenitor cells. It is produced by endothelial cells, monocytes, fibroblasts and T-lymphocytes. GM-CSF inhibits neutrophil migration and enhances the functional activity of the mature end-cells. Recombinant human GM-CSF was expressed in E. coli and purified by conventional chromatography, after refolding of the isolated inclusion bodies in a renaturation buffer.
Alternative Names: MGC131935 protein, GMCSF protein, Eosinophil Colony Stimulating Factor protein, CMCSF protein, Sargramostim protein, GM-CSF protein, Pluripoietin Alpha protein, Granulocyte Macrophage Colony Stimulating Factor protein, Colony-stimulating factor protein, , Colony Stimulating Factor 2 protein, CSF protein, Colony stimulating factor protein, CSF 2 protein, GM-CSF protein, CSF2 protein, CSF Alpha protein, Molgramostin protein, Granulocyte-macrophage colony-stimulating factor protein, MGC138897 protein, Granulocyte-macrophage colony stimulating factor Burst Promoting Activity protein

Molecular Weight: 14.6 kDa (128 AA)

Pathways: JAK-STAT Signaling, Cellular Response to Molecule of Bacterial Origin