TPMT Protein (AA 1-245)
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- Target See all TPMT Proteins
- TPMT (Thiopurine S-Methyltransferase (TPMT))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 1-245
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Origin
- Human
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Source
- Escherichia coli (E. coli)
- Application
- SDS-PAGE (SDS)
- Sequence
- MDGTRTSLDI EEYSDTEVQK NQVLTLEEWQ DKWVNGKTAF HQEQGHQLLK KHLDTFLKGK SGLRVFFPLC GKAVEMKWFA DRGHSVVGVE ISELGIQEFF TEQNLSYSEE PITEIPGTKV FKSSSGNISL YCCSIFDLPR TNIGKFDMIW DRGALVAINP GDRKCYADTM FSLLGKKFQY LLCVLSYDPT KHPGPPFYVP HAEIERLFGK ICNIRRLEKV DAFEERHKSW GIDCLFEKLY LLTEK
- Characteristics
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Purified recombinant Human TPMT protein
Expression System: E.coli
Molecular weight on SDS-PAGE will appear higher. - Purity
- > 95 % pure
- Top Product
- Discover our top product TPMT Protein
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Thiopurine S-Methyltransferase (TPMT) (AA 1-245) protein (GST tag)
TPMT Origin: Human Host: Wheat germ Recombinant ELISA, WB, AA, AP
Thiopurine S-Methyltransferase (TPMT) (AA 1-245) protein (Strep Tag)Crystallography grade TPMT Origin: Human Host: Tobacco (Nicotiana tabacum) Recombinant >80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, SDS, WB
Thiopurine S-Methyltransferase (TPMT) (AA 1-240) protein (Strep Tag)Crystallography grade TPMT Origin: Mouse Host: Tobacco (Nicotiana tabacum) Recombinant ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, SDS, WB
Thiopurine S-Methyltransferase (TPMT) (AA 4-244), (partial) protein (GST tag)TPMT Origin: Human Host: Escherichia coli (E. coli) Recombinant 95 % ELISA
Thiopurine S-Methyltransferase (TPMT) protein (Myc-DYKDDDDK Tag)TPMT Origin: Human Host: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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- Application Notes
- TPMT protein has been used in SDS PAGE and may be suitable for use in other assays to be determined by the end user.
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 1 mg/mL
- Buffer
- Supplied as a liquid in 20 mM Tris, pH 8.0, containing 0.0 mM PMSF, and 0 mM EDTA.
- Handling Advice
- Avoid repeated freeze/thaw cycles.
- Storage
- RT/-20 °C
- Storage Comment
- Store at 4 °C for short term storage (1/2 weeks). Aliquot and store at -20 °C or - 70 °C for long term storage.
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- Target
- TPMT (Thiopurine S-Methyltransferase (TPMT))
- Alternative Name
- TPMT (TPMT Products)
- Synonyms
- MGC84277 Protein, AW106912 Protein, NHLRC1 Protein, thiopurine S-methyltransferase L homeolog Protein, thiopurine S-methyltransferase, tandem duplicate 2 Protein, thiopurine S-methyltransferase TpmT Protein, thiopurine S-methyltransferase Protein, Thiopurine S-methyltransferase Protein, thiopurine methyltransferase Protein, tpmt.L Protein, tpmt.2 Protein, tpmT Protein, MCA0794 Protein, Pnap_4948 Protein, Hhal_1383 Protein, TK90_1044 Protein, Slit_1144 Protein, Tint_0842 Protein, Fbal_3334 Protein, Celly_3188 Protein, Weevi_1737 Protein, Psefu_2696 Protein, Metme_3860 Protein, Tpmt Protein, TPMT Protein
- Background
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TPMT, thiopurine S-methyltransferase, is a cytosolic enzyme that metabolizes thiopurine drugs via S-adenosyl-L-methionine as the S-methyl donor and S-adenosyl-L-homocysteine as a byproduct. Thiopurine drugs such as 6-mercaptopurine and azathioprine are used as chemotherapeutic agents. TPMT activity exhibits autosomal codominant genetic polymorphism, and patients inheriting TPMT-deficiency are at high risk of potentially fatal hematopoietic toxicity. Recombinant human TPMT was expressed in E. coli and purified by using conventional chromatography techniques.
Alternative Names: Thiopurine methyltransferase protein, Thiopurine S methyltransferase., Thiopurine S-methyltransferase HGNC:12014 protein, Thiopurine S-methyltransferase protein, TPMT protein, S adenosyl L methionine thiopurine S methyltransferase protein - Molecular Weight
- 28 kDa (245 AA)
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