PPIH Protein (AA 1-177)

Catalog No. ABIN934991

This Recombinant PPIH protein is produced in Escherichia coli (E. coli).

Quick Overview for PPIH Protein (AA 1-177) (ABIN934991)

Target: PPIH (Peptidylprolyl Isomerase H (Cyclophilin H) (PPIH))

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: Escherichia coli (E. coli)

Application: SDS-PAGE (SDS)

Purity: > 95 % pure

Product Details

Protein Characteristics: AA 1-177

Sequence: MAVANSSPVN PVVFFDVSIG GQEVGRMKIE LFADVVPKTA ENFRQFCTGE FRKDGVPIGY KGSTFHRVIK DFMIQGGDFV NGDGTGVASI YRGPFADENF KLRHSAPGLL SMANSGPSTN GCQFFITCSK CDWLDGKHVV FGKIIDGLLV MRKIENVPTG PNNKPKLPVV ISQCGEM

Characteristics: Purified recombinant Human PPIH protein
Expression System: E.coli
Bioactivity: Specific activity is > 40 nmoles/min/µg, and is defined as the amount of enzyme that cleaves 1uM of suc-AAFP-pNA per minute at 1 °C in Tris-HCl pH 8.0 using chymotrypsin.
Molecular weight on SDS-PAGE will appear higher.

Endotoxin Level: < 1.0 EU per µg of protein (determined by LAL method)

Application Details

Application Notes: PPIH protein has been used in SDS PAGE and may be suitable for use in other assays to be determined by the end user.

Assay Procedure:

1. Prepare assay buffer in a suitable container and pre-chill on ice before use: The final concentrations are 35 mM Tris, pH 8.0, 1 µM suc-AAFP-pNA).
   2. Add recombinant Cyclophilin H protein with various concentrations (1 µg, 1 µg, 1 µg) in assay buffer.
   3. Mix by inversion and equilibrate to 1 °C and monitor the A410 nm until the value is constant using a spectrophotometer.
   4. Add pre-chilled chymotrypsin to 12.5 µM and mix immediately.
   5. Record the increase in A410 nm for 20 minutes

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Supplied as a liquid in PBS, pH 7.4, containing 10 % glycerol.

Handling Advice: Avoid repeated freeze/thaw cycles.

Storage: RT/-20 °C

Storage Comment: Store at 4 °C for short term storage (1/2 weeks). Aliquot and store at -20 °C or - 70 °C for long term storage.

Target Details

Target: PPIH (Peptidylprolyl Isomerase H (Cyclophilin H) (PPIH))

Alternative Name: PPIH

Background: Cyclophilin H (also known as peptidylpropyl isomerase H, PPIH) is a member of peptidyl-propyl cis-trans isomerase (PPIase) family, which catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides and accelerates the folding of proteins. The cyclophilin H is a specific component of the human U4/U6 small nuclear ribonucleoprotein particle involved in the nuclear splicing of pre-mRNA. It stably associates with the U4/U6-60kD and -90kD proteins, the human orthologues of the Saccharomyces cerevisiae Prp4 and Prp3 splicing factors. Recombinant human cyclophilin H was expressed in E. coli and purified by conventional chromatography techniques.
Alternative Names: Peptidylprolyl isomerase H CypH protein, EC 5.2.1.8 protein, PPIH protein, UsnRNP associated cyclophilin SnuCyp20 protein, Peptidylprolyl isomerase H protein, Cyclophilin H protein, , CYPH protein, MGC5016 protein, CYP20 protein, USACYP protein, PPIase H protein, Rotamase H protein, SnuCyp-20 protein, SnuCyp-20 protein, Small nuclear ribonucleoprotein particle-specific cyclophilin H protein

Molecular Weight: 19.2 kDa (177 AA)