PPIH Protein (AA 1-177) (His tag)

Catalog No. ABIN1097379

Product Details

Target: PPIH (Peptidylprolyl Isomerase H (Cyclophilin H) (PPIH))

Protein Type: Recombinant

Protein Characteristics: AA 1-177

Origin: Human

Source: Escherichia coli (E. coli)

Purification tag / Conjugate: This PPIH protein is labelled with His tag.

Purpose: Recombinant Human Peptidyl-Prolyl Cis-Trans Isomerase H/PPIH (N-6His)

Sequence: MGSSHHHHHH SSGLVPRGSH MAVANSSPVN PVVFFDVSIG GQEVGRMKIE LFADVVPKTA ENFRQFCTGE FRKDGVPIGY KGSTFHRVIK DFMIQGGDFV NGDGTGVASI YRGPFADENF KLRHSAPGLL SMANSGPSTN GCQFFITCSK CDWLDGKHVV FGKIIDGLLV MRKIENVPTG PNNKPKLPVV ISQCGEM

Characteristics: Recombinant Human Peptidyl-Prolyl Cis-Trans Isomerase H/PPIH is produced by our E. coli expression system. The target protein is expressed with sequence (Met1-Met177) of Human PPIH fused with a His tag at the N-terminus.

Purity: > 95 % as determined by reducing SDS-PAGE.

Sterility: 0.2 μm filtered

Endotoxin Level: Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test

Application Details

Restrictions: For Research Use only

Handling

Format: Liquid

Reconstitution: It is not recommended to reconstitute to a concentration less than 100 μg/mL.
Dissolve the lyophilized protein in ddH2O.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

Buffer: Supplied as a 0.2 μm filtered solution of 20 mM PB, 150 mM NaCl, pH 7.2.

Handling Advice: Always centrifuge tubes before opening. Do not mix by vortex or pipetting.

Storage: -80 °C

Storage Comment: Store at < -20°C, stable for 6 months after receipt.
Please minimize freeze-thaw cycles.

Expiry Date: 6 months

Target Details

Target: PPIH (Peptidylprolyl Isomerase H (Cyclophilin H) (PPIH))

Alternative Name: PPIase-H (PPIH Products)

Synonyms: CYP-20 Protein, CYPH Protein, SnuCyp-20 Protein, USA-CYP Protein, RGD1564921 Protein, cyp-20 Protein, cyph Protein, snucyp-20 Protein, usa-cyp Protein, PPIH Protein, zgc:136730 Protein, zgc:158595 Protein, zgc:55766 Protein, zgc:86780 Protein, 1100001J08Rik Protein, 2010111B15Rik Protein, 4833408F11Rik Protein, AI464484 Protein, D4Wsu43e Protein, peptidylprolyl isomerase H Protein, peptidylprolyl isomerase H (cyclophilin H) Protein, peptidylprolyl isomerase H L homeolog Protein, peptidyl prolyl isomerase H Protein, PPIH Protein, Ppih Protein, ppih Protein, ppih.L Protein

Sub Type: Fusionprotein

Background: Peptidyl-Prolyl Cis-Trans Isomerase H (PPIH) belongs to the Cyclophilin-type PPIase family that accelerate the folding of proteins. PPIases can catalyze the cis-trans isomerization of Proline Imidic peptide bonds in oligopeptides. PPIH participates in pre-mRNA splicing. It is a specific component of the complex that includes pre-mRNA processing factors PRPF3, PRPF4, and PRPF18, as well as U4/U5/U6 tri-snRNP. In addition, PPIH has PPIase activity and may play a role as a chaperone mediating the interactions between different proteins inside the spliceosome.
Alternative Names: Peptidyl-Prolyl Cis-Trans Isomerase H, PPIase H, Rotamase H, Small Nuclear Ribonucleoprotein Particle-Specific Cyclophilin H, CypH, U-snRNP-Associated Cyclophilin SnuCyp-20, USA-CYP, PPIH, CYP20, CYPH

Molecular Weight: 21.4 kDa

UniProt: O43447