PPIL1 Protein (AA 1-166) (His tag)

Catalog No. ABIN935000

This Recombinant PPIL1 protein is produced in Escherichia coli (E. coli).

Quick Overview for PPIL1 Protein (AA 1-166) (His tag) (ABIN935000)

Target: PPIL1 (Peptidylprolyl Isomerase (Cyclophilin)-Like 1 (PPIL1))

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: Escherichia coli (E. coli)

Application: SDS-PAGE (SDS)

Purity: > 95 % pure

Product Details

Protein Characteristics: AA 1-166

Purification tag / Conjugate: This PPIL1 protein is labelled with His tag.

Sequence: MAAIPPDSWQ PPNVYLETSM GIIVLELYWK HAPKTCKNFA ELARRGYYNG TKFHRIIKDF MIQGGDPTGT GRGGASIYGK QFEDELHPDL KFTGAGILAM ANAGPDTNGS QFFVTLAPTQ WLDGKHTIFG RVCQGIGMVN RVGMVETNSQ DRPVDDVKII KAYPSGLEHH HHHH

Characteristics: Purified recombinant Human PPIL1 protein
Expression System: E.coli
Bioactivity: Specific activity is > 155 nM/min/µg, and is defined as the amount of enzyme that cleaves 1uM of suc-AAFP-pNA per minute at 1 °C in Tris-Hcl pH 8.0 using chymotrypsin.
Molecular weight on SDS-PAGE will appear higher.

Application Details

Application Notes: PPIL1 protein has been used in SDS PAGE and may be suitable for use in other assays to be determined by the end user.

Assay Procedure:

1. Prepare assay buffer in a suitable container and pre-chill on ice before use: The final concentrations are 35 mM Tris, pH 8.0, 1 µM suc-AAFP-pNA).
   2. Add recombinant PPIL1 protein with various concentrations (1 µg, 1 µg, 1 µg) in assay buffer.
   3. Mix by inversion and equilibrate to 1 °C and monitor the A410 nm until the value is constant using a spectrophotometer.
   4. Add pre-chilled chymotrypsin to 12.5 µM and mix immediately.
   5. Record the increase in A410 nm for 20 minutes

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Supplied as a liquid in 20 mM Tris-HCl, pH 8.0, containing 20 % glycerol.

Handling Advice: Avoid repeated freeze/thaw cycles.

Storage: RT/-20 °C

Storage Comment: Store at 4 °C for short term storage (1/2 weeks). Aliquot and store at -20 °C or - 70 °C for long term storage.

Target Details

Target: PPIL1 (Peptidylprolyl Isomerase (Cyclophilin)-Like 1 (PPIL1))

Alternative Name: PPIL1

Background: Peptidylprolyl isomerase (cyclophilin)-like 1, also known as PPIL1, is a member of peptidyl-propyl cis-trans isomerase (PPIase) family, which catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides and accelerates the folding of proteins. Human PPIL1 might play an important role in proliferation of cancer cells through modulation of phosphorylation of stathmin. So it is expected as a novel molecular target for colon-cancer therapy. Recombinant PPIL1, fused to His-tag at C-terminus, was expressed in E. coli and purified by conventional chromatography techniques.
Alternative Names: PPIL 1, Cyclophilin related gene 1 protein, PPIL-1, Peptidylprolyl isomerase (cyclophilin)-like 1 CGI124 protein, Peptidylprolyl isomerase (cyclophilin) like 1 protein, CYPL1 protein, PPIL-1 protein, rotamase0., PPIL1, PPIL 1 protein, PPIase protein, PPIL1 protein, peptidylprolyl isomerase like 1 protein, Peptidylprolyl isomerase (cyclophilin)-like 1 protein, CGI-124 protein, MGC678 protein, hCyPX protein

Molecular Weight: 19.3 kDa (174 AA)