Glyoxalase Protein (AA 1-184)
Origin: Human
Host: Escherichia coli (E. coli)
Recombinant
> 90 % pure
SDS
Active
1 image
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- Target
- Glyoxalase
- Protein Type
- Recombinant
- Biological Activity
- Active
- Protein Characteristics
- AA 1-184
- Origin
- Human
- Source
- Escherichia coli (E. coli)
- Application
- SDS-PAGE (SDS)
- Sequence
- MAEPQPPSGG LTDEAALSCC SDADPSTKDF LLQQTMLRVK DPKKSLDFYT RVLGMTLIQK CDFPIMKFSL YFLAYEDKND IPKEKDEKIA WALSRKATLE LTHNWGTEDD ETQSYHNGNS DPRGFGHIGI AVPDVYSACK RFEELGVKFV KKPDDGKMKG LAFIQDPDGY WIEILNPNKM ATLM
- Characteristics
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Purified recombinant Human Glyoxalase? protein
Expression System: E.coli
Bioactivity: Specific activity: >0.4 units/mg (please enquire for specific batch value). One unit will form 1.0 umol of S-lactoylglutathione from methylglyoxal and reduced glutathione per minute at pH 7.5, at 25 °C.
Molecular weight on SDS-PAGE will appear higher. - Purity
- > 90 % pure
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- Application Notes
- Glyoxalase I protein has been used in SDS PAGE and may be suitable for use in other assays to be determined by the end user.
- Assay Procedure
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- Prepare a 1.5 mL reaction mix into a suitable container: The final concentrations are 79 mM potassium phosphate, 0.033 % (w/v) reduced glutathine, 0.003 % (w/v) bovine serum albumin.
2. Equilibrate to 25 °C and monitor at A240 nm (absorbance) until the value is constant, using a spectrophotometer.
3. Add 50 µL of recombinant glyoxalase I solution with various concentrations (0.5 µg, 5 µg, 5 µg) in 1.5 mL reaction buffer.
4. Immediately mix by inversion and record the increase at A240 nm for 5 minutes.
- Prepare a 1.5 mL reaction mix into a suitable container: The final concentrations are 79 mM potassium phosphate, 0.033 % (w/v) reduced glutathine, 0.003 % (w/v) bovine serum albumin.
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 1 mg/mL
- Buffer
- Supplied as a liquid in 20 mM Tris-HCl buffer, pH 8.0, containing 0 mM DTT, and 10 % glycerol.
- Preservative
- Dithiothreitol (DTT)
- Precaution of Use
- This product contains Dithiothreitol: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.
- Handling Advice
- Avoid repeated freeze/thaw cycles.
- Storage
- RT/-20 °C
- Storage Comment
- Store at 4 °C for short term storage (1/2 weeks). Aliquot and store at -20 °C or - 70 °C for long term storage.
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- Target
- Glyoxalase
- Alternative Name
- Glyoxalase?
- Background
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Glyoxalase I, also known as GLO1, belongs to the glyoxalase family. Glyoxalase I is responsible for the catalysis and formation of S-lactoyl-glutathione from methylglyoxal condensation and reduced glutathione.This enzyme is ubiquitously expressed and is also present in many tumor cell lines, in which its concentration is often upregulated. Recombinant human GLO1 protein was expressed in E. coli and purified by using conventional chromatography techniques.
Alternative Names: GLO-1 Methylglyoxalase protein, Lactoylglutathione lyase protein, Lactoyl glutathione lyase protein, Ketone aldehyde mutase protein, Glx 1 protein, GLOD1 protein, S D lactoylglutathione methylglyoxal lyase 0., Glyoxalase I protein, GLYI protein, Glx I protein, Aldoketomutase protein - Molecular Weight
- 20.7 kDa (184 AA)
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