GFP Protein (AA 1-238)

Catalog No. ABIN935030

This Recombinant GFP protein is produced in Escherichia coli (E. coli).

Quick Overview for GFP Protein (AA 1-238) (ABIN935030)

Target: GFP (Green Fluorescent Protein (GFP))

Protein Type: Recombinant

Origin: Aequorea victoria

Source: Escherichia coli (E. coli)

Application: SDS-PAGE (SDS)

Purity: > 95 % pure

Product Details

Protein Characteristics: AA 1-238

Sequence: MSKGEELFTG VVPILVELDG DVNGHKFSVS GEGEGDATYG KLTLKFICTT GKLPVPWPTL VTTFSYGVQC FSRYPDHMKQ HDFFKSAMPE GYVQERTIFF KDDGNYKTRA EVKFEGDTLV NRIELKGIDF KEDGNILGHK LEYNYNSHNV YIMADKQKNG IKVNFKIRHN IEDGSVQLAD HYQQNTPIGD GPVLLPDNHY LSTQSALSKD PNEKRDHMVL LEFVTAAGIT HGMDELYK

Characteristics: Purified recombinant Aequorea victoria GFP protein
Expression System: E.coli
Molecular weight on SDS-PAGE will appear higher.

Endotoxin Level: < 1.0 EU per µg of protein (determined by LAL method)

Application Details

Application Notes: GFP protein has been used in SDS PAGE and may be suitable for use in other assays to be determined by the end user.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Supplied as a liquid in 20 mM Tris-HCl buffer, pH 8.0, containing 10 % glycerol.

Handling Advice: Avoid repeated freeze/thaw cycles.

Storage: RT/-20 °C

Storage Comment: Store at 4 °C for short term storage (1/2 weeks). Aliquot and store at -20 °C or - 70 °C for long term storage.

Target Details

Target: GFP (Green Fluorescent Protein (GFP))

Alternative Name: GFP

Background: GFP, also known as green fluorescent protein, is a protein produced by the jellyfish that emits bioluminescence in the green zone of the visible spectrum. GFP has become a useful and ubiquitous tool for making chimeric proteins, where it functions as a fluorescent protein tag. It has been expressed in most known cell types and is used as a noninvasive fluorescent marker in living cells and organisms. This protein enables a wide range of applications where it has functioned as a cell lineage tracer, reporter of gene expression, or as a measure of protein-protein interactions. Recombinant GFP protein was expressed in E. coli and purified by using conventional chromatography.
Alternative Names: Green fluorescent protein

Molecular Weight: 26.8 kDa (238 AA)