Ataxin 3 Protein (ATXN3) (AA 1-370)

Catalog No. ABIN935045

This Recombinant Ataxin 3 protein is produced in Escherichia coli (E. coli).

Quick Overview for Ataxin 3 Protein (ATXN3) (AA 1-370) (ABIN935045)

Target: Ataxin 3 (ATXN3)

Protein Type: Recombinant

Origin: Human

Source: Escherichia coli (E. coli)

Application: SDS-PAGE (SDS)

Purity: > 90 % pure

Product Details

Protein Characteristics: AA 1-370

Sequence: MESIFHEKQE GSLCAQHCLN NLLQGEYFSP VELSSIAHQL DEEERMRMAE GGVTSEDYRT FLQQPSGNMD DSGFFSIQVI SNALKVWGLE LILFNSPEYQ RLRIDPINER SFICNYKEHW FTVRKLGKQW FNLNSLLTGP ELISDTYLAL FLAQLQQEGY SIFVVKGDLP DCEADQLLQM IRVQQMHRPK LIGEELAQLK EQRVHKTDLE RVLEANDGSG MLDEDEEDLQ RALALSRQEI DMEDEEADLR RAIQLSMQGS SRNISQDMTQ TSGTNLTSEE LRKRREAYFE KQQQKQQQQQ QQQQQQQQQQ QQQQGDLSGQ SSHPCERPAT SSGALGSDLG DAMSEEDMLQ AAVTMSLETV RNDLKTEGKK

Characteristics: Purified recombinant Human Ataxin 3 protein
Expression System: E.coli
Molecular weight on SDS-PAGE will appear higher.

Endotoxin Level: < 1.0 EU per µg of protein (determined by LAL method)

Application Details

Application Notes: Ataxin protein has been used in SDS PAGE and may be suitable for use in other assays to be determined by the end user.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Supplied as a liquid in 20 mM Tris-HCl buffer, pH 7.5, containing 0 mM DTT, 50 mM NaCl, and 10 % glycerol.

Preservative: Dithiothreitol (DTT)

Precaution of Use: This product contains Dithiothreitol: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze/thaw cycles.

Storage: RT/-20 °C

Storage Comment: Store at 4 °C for short term storage (1/2 weeks). Aliquot and store at -20 °C or - 70 °C for long term storage.

Target Details

Target: Ataxin 3 (ATXN3)

Alternative Name: Ataxin 3

Background: Ataxin 3 is also known as Machado-Joseph disease protein 1. Machado-Joseph disease is one of several hereditary autosomal dominant neurodegenerative disorders. This protein contains trinucleotide CAG repeats in the coding region, and the expansion of these repeats from the normal 13-36 to 68-79 is the cause of Machado-Joseph disease. Ataxin 3 interacts with the major histone acetyltransferases cAMP-response-element binding protein (CREB)-binding protein, p300, and p300/CREB-binding protein-associated factor and inhibits transcription by these coactivators. Also, ataxin-3 is a proteasome-associated factor that mediates the degradation of ubiquitinated proteins. Recombinant human Ataxin-3 was expressed in E. coli and purified by using conventional chromatography.
Alternative Names: Machado-Joseph disease protein 1 homolog protein, Machado Joseph disease protein, AT3 protein, JOS protein, MJD1 protein, MJD gene protein, Machado Joseph disease protein 1 protein, EC 3.4.22. protein, ATXN3 protein, , Spinocerebellar ataxia type 3 protein protein, Rsca3 protein, MJD protein, Josephin protein, ATX3 protein, ATXN3 protein, SCA3 protein, Ataxin 3 protein, SCA3 gene protein

Molecular Weight: 42.4 kDa (370 AA)