APRT Protein (AA 1-180)
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- Target See all APRT Proteins
- APRT (Adenine Phosphoribosyltransferase (APRT))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 1-180
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Origin
- Human
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Source
- Escherichia coli (E. coli)
- Application
- SDS-PAGE (SDS)
- Sequence
- MADSELQLVE QRIRSFPDFP TPGVVFRDIS PVLKDPASFR AAIGLLARHL KATHGGRIDY IAGLDSRGFL FGPSLAQELG LGCVLIRKRG KLPGPTLWAS YSLEYGKAEL EIQKDALEPG QRVVVVDDLL ATGGTMNAAC ELLGRLQAEV LECVSLVELT SLKGREKLAP VPFFSLLQYE
- Characteristics
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Purified recombinant Human APRT protein
Expression System: E.coli - Purity
- > 90 % pure
- Top Product
- Discover our top product APRT Protein
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Adenine Phosphoribosyltransferase (APRT) (AA 1-180) protein (GST tag)
APRT Origin: Human Host: Wheat germ Recombinant WB, ELISA, AA, AP
Adenine Phosphoribosyltransferase (APRT) (AA 2-180) protein (His tag)Crystallography grade APRT Origin: Human Host: Escherichia coli (E. coli) Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA, Crys
Adenine Phosphoribosyltransferase (APRT) (AA 2-180) protein (His tag)Crystallography grade APRT Origin: Mouse Host: Escherichia coli (E. coli) Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA, Crys
Adenine Phosphoribosyltransferase (APRT) (AA 1-180) (Active) protein (His tag)APRT Origin: Human Host: Escherichia coli (E. coli) > 97 % AcA, CC Active
Adenine Phosphoribosyltransferase (APRT) (Transcript Variant 1) protein (Myc-DYKDDDDK Tag)APRT Origin: Human Host: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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- Application Notes
- APRT protein has been used in SDS PAGE and may be suitable for use in other assays to be determined by the end user.
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 1 mg/mL
- Buffer
- Supplied as a liquid in 20 mM Tris-HCl buffer, pH 8.0, containing 0 mM DTT and 10 % glycerol.
- Preservative
- Dithiothreitol (DTT)
- Precaution of Use
- This product contains Dithiothreitol: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.
- Handling Advice
- Avoid repeated freeze/thaw cycles.
- Storage
- RT/-20 °C
- Storage Comment
- Store at 4 °C for short term storage (1/2 weeks). Aliquot and store at -20 °C or - 70 °C for long term storage.
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- Target
- APRT (Adenine Phosphoribosyltransferase (APRT))
- Alternative Name
- APRT (APRT Products)
- Synonyms
- Tb07.43M14.200 Protein, Tb07.43M14.180 Protein, C85684 Protein, AMP Protein, APRTD Protein, adenine phosphoribosyltransferase Protein, adenine phosphoribosyl transferase Protein, CND05020 Protein, Tb927.7.1790 Protein, Tb927.7.1780 Protein, Arnit_0941 Protein, Saut_1231 Protein, Fbal_1180 Protein, PH_RS07970 Protein, PF_RS08760 Protein, PAB_RS02560 Protein, Aprt Protein, APRT Protein
- Background
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APRT (adenine phosphoribosyltransferase) is a 180 amino acid protein that localizes to the cytoplasm and belongs to the purine/pyrimidine phosphoribosyltransferase family. Existing as a homodimer, APRT functions to catalyze the formation of inorganic pyrophosphate and AMP from adenine and 5-phosphoribosyl-1-pyrophosphate (PRPP), a reaction that is essential for both purine metabolism and AMP biosynthesis. It also produces adenine as a by-product of the polyamine biosynthesis pathway. Recombinant human APRT protein was expressed in E. coli and purified by using conventional chromatography techniques.
Alternative Names: AMP pyrophosphorylase protein, MGC125856 protein, Adenine phosphoribosyltransferase AMP diphosphorylase protein, DKFZp686D13177 protein, MGC129961 protein, Transphosphoribosidase., AMP protein, MGC125857 protein - Molecular Weight
- 19.6 kDa (180 AA)
- Pathways
- Ribonucleoside Biosynthetic Process
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