CNTF Protein
Quick Overview for CNTF Protein (ABIN935291)
Target
See all CNTF ProteinsProtein Type
Biological Activity
Origin
Source
Purity
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Sequence
- AFAEQTPLTL HRRDLCSRSI WLARKIRSDL TALMESYVKH QGLNKNINLD SVDGVPVAST DRWSEMTEAE RLQENLQAYR TFQGMLTKLL EDQRVHFTPT EGDFHQAIHT LMLQVSAFAY QLEELMVLLE QKIPENEADG MPATVGDGGL FEKKLWGLKV LQELSQWTVR SIHDLRVISS HQMGISALES HYGAKDKQM
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Characteristics
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Purified recombinant Human CNTF protein
Expression System: E.coli
Bioactivity: Determined by its ability to stimulate proliferation of human TF-1 cells using a concentration range of 25.0-35.0 ng/mL. -
Endotoxin Level
- < 0.1 ng per µg (1 EU/µg).
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Ciliary Neurotrophic Factor (CNTF) (AA 1-200) protein (His tag)
custom-made CNTF Origin: Human Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
Ciliary Neurotrophic Factor (CNTF) (AA 1-198) protein (His tag)custom-made CNTF Origin: Mouse Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
Ciliary Neurotrophic Factor (CNTF) proteinCNTF Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 95 % as determined by reducing SDS-PAGE.
Ciliary Neurotrophic Factor (CNTF) (AA 1-200) protein (His tag)CNTF Origin: Rat Host: Escherichia coli (E. coli) Recombinant Greater than 95 % as determined by SDS-PAGE WB, ELISA
Ciliary Neurotrophic Factor (CNTF) (AA 1-200) protein (His tag)CNTF Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 95 % as determined by SDS-PAGE WB, ELISA
Ciliary Neurotrophic Factor (CNTF) (AA 1-200) protein (GST tag)CNTF Origin: Human Host: Wheat germ Recombinant WB, ELISA, AP, AA
Ciliary Neurotrophic Factor (CNTF) (AA 1-200) (Active) proteinCNTF Origin: Human Host: Escherichia coli (E. coli) Recombinant > 90 % by SDS - PAGE SDS Active
Ciliary Neurotrophic Factor (CNTF) (AA 1-200) (Active) protein (His tag)CNTF Origin: Human Host: Escherichia coli (E. coli) Recombinant > 95 % by SDS - PAGE SDS Active
Ciliary Neurotrophic Factor (CNTF) (AA 4-196), (partial) protein (His tag)CNTF Origin: Human Host: Escherichia coli (E. coli) Recombinant 90 % ELISA
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Application Notes
- Each Investigator should determine their own optimal working dilution for specific applications.
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Restrictions
- For Research Use only
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Format
- Lyophilized
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Reconstitution
- Reconstitute in 5 M Tris, pH 8.0 to a concentration of 0.1-1.0 mg/mL.
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Buffer
- Lyophilized from 5 mM tris, pH 8.0.
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Handling Advice
- Avoid repeated freeze/thaw cycles.
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Storage
- 4 °C/-20 °C
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Storage Comment
- Store at 4 °C until reconstitution. Following reconstitution aliquot and freeze at -20 °C for long term storage.
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- CNTF (Ciliary Neurotrophic Factor (CNTF))
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Alternative Name
- CNTF
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Background
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CNTF is a potent neural factor that was originally characterized as a vital factor for the survival of chick ciliary neurons in vitro. CNTF is also important for the survival of other neural cell types including primary sensory neurons, motor neurons, basal forebrain neurons and type 2 astrocytes. CNTF is highly conserved across species and exhibits cross-species bioactivity.
Alternative Names: Ciliary Neurotrophic Factor protein -
Molecular Weight
- 22.7 kDa
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Pathways
- JAK-STAT Signaling
Target
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