EGF Protein
Quick Overview for EGF Protein (ABIN935319)
Target
See all EGF ProteinsProtein Type
Biological Activity
Origin
Source
Purity
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Characteristics
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Purified recombinant Human EGF protein
Expression System: E.coli
Bioactivity: The ED50, calculated by the dose-dependant proliferation of murine BALB/c 3T3 cells (measured by 3H-thymidine uptake) is
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Epidermal Growth Factor (EGF) (AA 971-1023) protein
EGF Origin: Human Host: Escherichia coli (E. coli) Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Epidermal Growth Factor (EGF) (Active) proteinEGF Origin: Human Host: Escherichia coli (E. coli) > 95 % as determined by (a) Analysis by SEC-HPLC (b) Analysis by reducing and non-reducing SDS-PAGE Silver Stained gel Active
Epidermal Growth Factor (EGF) (AA 971-1023) protein (Fc Tag)EGF Origin: Human Host: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Epidermal Growth Factor (EGF) (AA 977-1029) protein (Fc Tag)EGF Origin: Mouse Host: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Epidermal Growth Factor (EGF) (AA 971-1023) (Active) protein (Fc Tag)EGF Origin: Human Host: HEK-293 Cells Recombinant >95 % as determined by SDS-PAGE. Active
Epidermal Growth Factor (EGF) (Active) proteinEGF Origin: Mouse Host: Escherichia coli (E. coli) Recombinant >95 % as determined by SDS-PAGE Func Active
Epidermal Growth Factor (EGF) (AA 974-1026) protein (His tag)EGF Origin: Rat Host: Escherichia coli (E. coli) Recombinant Greater than 95 % as determined by SDS-PAGE WB, ELISA
Epidermal Growth Factor (EGF) (AA 926-1025) protein (GST tag)EGF Origin: Human Host: Wheat germ Recombinant WB, ELISA, AA, AP
Epidermal Growth Factor (EGF) (AA 977-1029) (Active) protein (His tag)EGF Origin: Mouse Host: Escherichia coli (E. coli) Recombinant > 95% by SDS-PAGE SDS, AcA Active
Epidermal Growth Factor (EGF) proteinEGF Origin: Mouse Host: Escherichia coli (E. coli) Recombinant 97,00% SDS
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Application Notes
- Each Investigator should determine their own optimal working dilution for specific applications.
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Assay Procedure
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EGF quantitation was carried out by two independent methods 1. UV spectroscopy at 280 nm using the absorbency value of
2.858 as the extinction coefficient for a 0.1 % (1 mg/mL) solution.
2. Analysis by RP-HPLC, using a calibrated solution of EGF as a Reference Standard. -
Restrictions
- For Research Use only
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Format
- Lyophilized
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Reconstitution
- Reconstitute the lypholized rHuEGF in sterile water not less than 100 µg/mL.
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Buffer
- Supplied as a lyophilized powder.
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Handling Advice
- Avoid repeated freeze/thaw cycles.
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Storage
- RT/-20 °C
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- EGF (Epidermal Growth Factor (EGF))
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Alternative Name
- EGF
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Background
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Epidermal growth factor has a profound effect on the differentiation of specific cells in vivo and is a potent mitogenic factor for a variety of cultured cells of both ectodermal and mesodermal origin. The EGF precursor is believed to exist as a membrane-bound molecule which is proteolytically cleaved to generate the 53-amino acid peptide hormone that stimulates cells to divide. EGF stimulates the growth of various epidermal and epithelial tissues in vivo and in vitro and of some fibroblasts in cell culture.
Alternative Names: Epidermal Growth Factor protein, Urogastrone protein, URG protein -
Pathways
- NF-kappaB Signaling, RTK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Regulation of Carbohydrate Metabolic Process, Hepatitis C, Protein targeting to Nucleus, Interaction of EGFR with phospholipase C-gamma, Thromboxane A2 Receptor Signaling, EGFR Downregulation
Target
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