FGF acidic (Active) Protein

Catalog No. ABIN935349

This Recombinant protein is expressed in Escherichia coli (E. coli).

Quick Overview for FGF acidic (Active) Protein (ABIN935349)

Target: FGF acidic

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: Escherichia coli (E. coli)

Purity: > 95 % pure

Product Details

Sequence: MFNLPPGNYK KPKLLYCSNG GHFLRILPDG TVDGTRDRSD QHIQLQLSAE SVGEVYIKST ETGQYLAMDT DGLLYGSQTP NEECLFLERL EENHYNTYIS KKHAEKNWFV GLKKNGSCKR GPRTHYGQKA ILFLPLPVSS D

Characteristics: Purified recombinant Human FGF acidic protein
Expression System: E.coli
Bioactivity: Assay #1: The ED50 as determined by the dose-dependent stimulation of thymidine uptake by BaF3 cells expressing FGF receptors is ? 10 ng/mL, corresponding to a specific activity of ? 1 x 10^5 units/mg. Assay #2: The ED50 was determined by a cell proliferation assay using balb/c 3T3 cells is ? 0.5 ng/mL in the presence of 10 µg/mL heparin, corresponding to a specific activity of ? 2 x 10^6 units/mg.

Endotoxin Level: < 0.1 ng per μg (1 EU/μg).

Application Details

Application Notes: Each Investigator should determine their own optimal working dilution for specific applications.

Comment:

Biological activity: Assay #1: The ED50 as determined by the dose-dependent stimulation of thymidine uptake by BaF3 cells expressing FGF receptors is approx. 10 ng/mL, corresponding to a specific activity of approx. 1 x 10^5 units/mg. Assay #2: The ED50 was determined by a cell proliferation assay using balb/c 3T3 cells is approx. 0.5 ng/mL in the presence of 10 μg/mL heparin, corresponding to a specific activity of approx. 2 x 10^6 units/mg.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Reconstitute in 5 M Na3PO4

Buffer: Lyophilized from 5 mM Na3PO4, pH 8.0 with 105 mM NaCl.

Handling Advice: Avoid repeated freeze/thaw cycles.

Storage: RT/-20 °C

Target Details

Target: FGF acidic

Background: FGF acidic is one of 23 known members of the FGF family. Proteins of this family play a central role during prenatal development and postnatal growth and regeneration of a variety of tissues, by promoting cellular proliferation and differentiation. FGF acidic is a non-glycosylated heparin binding growth factor that is expressed in the brain, kidney, retina, smooth muscle cells, bone matrix, osteoblasts, astrocytes and endothelial cells. FGF acidic has the ability to signal through all the FGF receptors.
Alternative Names: HBGF-1 protein, FGF-1 protein, Fibroblast Growth Factor-acidic protein, ECGF-beta protein

Molecular Weight: 15.8 kDa