FASL Protein
Quick Overview for FASL Protein (ABIN935363)
Target
See all FASL ProteinsProtein Type
Biological Activity
Origin
Source
Purity
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Sequence
- HHHHHHHHPS PPPEKKELRK VAHLTGKSNS RSMPLEWEDT YGIVLLSGVK YKKGGLVINE TGLYFVYSKV YFRGQSCNNL PLSHKVYMRN SKYPQDLVMM EGKMMSYCTT GQMWARSSYL GAVFNLTSAD HLYVNVSELS LVNFEESQTF FGLYKL
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Characteristics
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Purified soluble Human FasL protein
Expression System: CHO cells
Bioactivity: Determined by it's ability to induce cytotoxicity in Jurkat cells in the absence of any cross-linking. The ED50 for this effect is ? 10.0 ng/mL, corresponding to a specific activity of ? 1 x 10^5 units/mg. -
Endotoxin Level
- < 0.1 ng per µg (1 EU/µg).
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Fas Ligand (TNF Superfamily, Member 6) (FASL) (AA 134-281) (Active) protein (His tag)
FASL Origin: Human Host: HEK-293 Cells Recombinant > 95 % as determined by Bis-Tris PAGE Active
Fas Ligand (TNF Superfamily, Member 6) (FASL) (AA 1-281) protein (GST tag)FASL Origin: Human Host: Wheat germ Recombinant WB, ELISA, AP, AA
Fas Ligand (TNF Superfamily, Member 6) (FASL) protein (His tag)FASL Origin: Human Host: Human Cells Recombinant >95 % as determined by reducing SDS-PAGE.
Fas Ligand (TNF Superfamily, Member 6) (FASL) (AA 99-330) protein (Fc Tag)FASL Origin: Human Host: HEK-293 Cells Recombinant The purity of the protein is greater than 95 % as determined by SDS-PAGE and Coomassie blue staining. ScA, IA, ADA, AM
Fas Ligand (TNF Superfamily, Member 6) (FASL) proteinFASL Origin: Human Host: Escherichia coli (E. coli) Recombinant > 90 % pure SDS
Fas Ligand (TNF Superfamily, Member 6) (FASL) (AA 134-281) (Active) protein (His tag)FASL Origin: Human Host: HEK-293 Cells Recombinant >90 % as determined by SDS-PAGE. Active
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Application Notes
- Each Investigator should determine their own optimal working dilution for specific applications.
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Restrictions
- For Research Use only
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Format
- Lyophilized
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Buffer
- Supplied as a lyophilized powder.
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Handling Advice
- Avoid repeated freeze/thaw cycles.
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Storage
- RT/-20 °C
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- FASL (Fas Ligand (TNF Superfamily, Member 6) (FASL))
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Alternative Name
- FasL
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Background
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Fas and Fas Ligand (FasL) belong to the TNF superfamily and are type I and type II transmembrane proteins, respectively. Binding of FasL to Fas triggers apoptosis in Fas-bearing cells. The mechanism of apoptosis involves recruitment of pro-caspase 8 through an adaptor molecule called FADD followed by processing of the pro-enzyme to active forms. These active caspases then cleave various cellular substrates leading to the eventual cell death. sFasR is capable of inhibiting FasL-induced apoptosis by acting as a decoy receptor that serves as a sink for FasL. The full length Fas (receptor) is a 319 amino acid type I transmembrane protein, which contains a 157 amino acid extracellular domain, a 17 amino acid transmembrane domain, and 145 amino acid cytoplasmic domain.
Alternative Names: Apo I Ligand protein, CD95L protein, TNFSF6 protein, soluble Fas Ligand protein, APTL protein, sFASL protein -
Molecular Weight
- 17.6 kDa
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Pathways
- Apoptosis, EGFR Signaling Pathway, Production of Molecular Mediator of Immune Response, Positive Regulation of Endopeptidase Activity
Target
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