IL-3 Protein
Quick Overview for IL-3 Protein (ABIN935532)
Target
See all IL-3 ProteinsProtein Type
Biological Activity
Origin
Source
Purity
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Characteristics
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Purified recombinant Human IL3 protein
Expression System: E.coli
Bioactivity: The ED50 as determined by the dose-dependant stimulation of TF-1 cells is
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Interleukin 3 (IL-3) (AA 20-152) protein (His-Avi Tag)
IL-3 Origin: Human Host: Escherichia coli (E. coli) Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Interleukin 3 (IL-3) (AA 20-152) protein (Fc Tag)IL-3 Origin: Human Host: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Interleukin 3 (IL-3) (AA 20-143) protein (His tag)IL-3 Origin: Cynomolgus Host: Escherichia coli (E. coli) Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Interleukin 3 (IL-3) protein (His tag)IL-3 Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 95 % as determined by reducing SDS-PAGE.
Interleukin 3 (IL-3) (AA 20-152) protein (His-Avi Tag,Biotin)IL-3 Origin: Human Host: Escherichia coli (E. coli) Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Interleukin 3 (IL-3) (AA 27-166) protein (Fc Tag)IL-3 Origin: Mouse Host: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Interleukin 3 (IL-3) (AA 33-166) (Active) proteinIL-3 Origin: Mouse Host: Escherichia coli (E. coli) Recombinant > 95 % by SDS-PAGE analysis. Active
Interleukin 3 (IL-3) (AA 20-152) (Active) proteinIL-3 Origin: Human Host: Escherichia coli (E. coli) Recombinant > 95 % as analyzed by SDS-PAGE and HPLC. Active
Interleukin 3 (IL-3) protein (His tag)IL-3 Origin: Mouse Host: Human Cells Recombinant > 95 % as determined by SDS-PAGE
Interleukin 3 (IL-3) (AA 1-152) protein (GST tag)IL-3 Origin: Human Host: Wheat germ Recombinant WB, ELISA, AA, AP
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Application Notes
- Each Investigator should determine their own optimal working dilution for specific applications.
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Assay Procedure
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Protein quantitation was carried out by two independent methods 1. UV spectroscopy at 280 nm using the absorbency value of 0.84 as the extinction coefficient for a 0.1 % (1 mg/mL) solution.
2. Analysis by RP-HPLC, using a standard solution of IL-3 as a Reference Standard. -
Restrictions
- For Research Use only
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Format
- Lyophilized
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Reconstitution
- Reconstitute in sterile water to a concentration of at least 100 µg/mL.
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Buffer
- Lyophilized from a concentrated (1 mg/mL) solution in water containing 5 mg HSA and 4.3 mg NaCl.
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Handling Advice
- Avoid repeated freeze/thaw cycles.
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Storage
- RT/-20 °C
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- IL-3 (Interleukin 3 (IL-3))
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Alternative Name
- IL3
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Background
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IL3 is a potent growth promoting cytokine. This cytokine is capable of supporting the proliferation of a broad range of hematopoietic cell types. It is involved in a variety of cell activities such as cell growth, differentiation and apoptosis. This cytokine has been shown to also possess neurotrophic activity, and it may be associated with neurologic disorders.
Alternative Names: P-cell stimulation factor protein, Interleukin 3 protein, Multi-CSF protein, P-cell stimulation factor protein, IL-3 protein, Multi-CSF protein, HCGF protein, MCGF protein, IL-3 protein, IL-3 protein, IL-3, IL3, IL 3, MCGF (Mast cell growth factor) protein, MGC79399, HCGF protein, Mast cell growth factor protein, IL 3 protein, MGC79398 protein -
Molecular Weight
- 15,000 Da
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Pathways
- JAK-STAT Signaling, Regulation of Carbohydrate Metabolic Process, Autophagy
Target
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