IL-21 Protein

Catalog No. ABIN935561

Product Details

Target: IL-21 (IL21) (Interleukin 21 (IL21))

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: Escherichia coli (E. coli)

Characteristics: Purified recombinant Human IL21 protein
Expression System: E.coli
Bioactivity: ED50 = 1.0-10 ng/mL, determined by the dose-dependant proliferation of human peripheral blood mononuclear cells.

Purity: > 95 % pure

Application Details

Application Notes: Each Investigator should determine their own optimal working dilution for specific applications.

Assay Procedure:

Protein quantitation was carried out by two independent methods. 1. UV spectroscopy at 280 nm using the absorbency value of 0.54 as the extinction coefficient for a 0.1 % (1 mg/mL) solution.
2. Analysis by RP-HPLC, using a standard solution of IL-21 as a Reference Standard.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Buffer: Supplied as a lyophilized powder.

Handling Advice: Avoid repeated freeze/thaw cycles.

Storage: RT/-20 °C

Target Details

Target: IL-21 (IL21) (Interleukin 21 (IL21))

Alternative Name: IL21 (IL21 Products)

Background: IL-21 is produced by CD4+ T cells in response to antigenic stimulation. Its action enhances antigen-specific responses of immune cells. The biological effects of IL-21 include induction of differentiation of T-cells-stimulated B-cells into plasma cells and memory B-cells, stimulation (in conjuction) with IL-4 of IgG production, and induction of apoptotic effects in na?ve B-cells and stimulated B-cells in the absence of T-cell signaling. Additionally, IL-21 promotes the anti-tumor activity of CD8+ T-cells and NK cells. IL-21 exerts its effect through binding to a specific type I cytokine receptor, IL-21R, which also contains the gamma chain (°C) found in other cytokine receptors including IL-2, IL-4, IL-7, IL-9 and IL-15. The IL-21/IL-21R interaction triggers a cascade of events which includes activation of the tyrosine kinases JAK1 and JAK3, followed by activation of the transcription factors STAT1 and STAT3.
Alternative Names: Za11 protein, Interleukin 21 protein, IL 21, IL-21, IL 21 protein, IL-21 protein, IL-21 protein, IL-21 protein, IL21

Molecular Weight: 15,463 Da

Pathways: JAK-STAT Signaling, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response