ALOX5 Protein
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- Target See all ALOX5 Proteins
- ALOX5 (Arachidonate 5-Lipoxygenase (ALOX5))
- Protein Type
- Recombinant
- Biological Activity
- Active
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Origin
- Human
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Source
- Baculovirus
- Characteristics
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Purified recombinant Human 5-Lipoxygenase protein
Expression System: Baculovirus
Bioactivity: 20 units/mg protein - One unit is defined as the amount of enzyme that consumes 1.0 nmol of oxygen per min at 25 °C in 50 mM Tris-HCl, 100 uM arachidonate, 2 mM CaCl2, 1 mM ATP, 250 uM oxygen. Oxygen consumption can be measured using a oxygraph equipped with a Clark oxygen electrode. - Top Product
- Discover our top product ALOX5 Protein
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Arachidonate 5-Lipoxygenase (ALOX5) (AA 1-674) protein (GST tag)
ALOX5 Origin: Human Host: Wheat germ Recombinant WB, ELISA, AA, AP
Arachidonate 5-Lipoxygenase (ALOX5) (AA 1-674) protein (Strep Tag)Crystallography grade ALOX5 Origin: Human Host: Tobacco (Nicotiana tabacum) Recombinant >80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA
Arachidonate 5-Lipoxygenase (ALOX5) (AA 1-674) protein (Strep Tag)Crystallography grade ALOX5 Origin: Mouse Host: Tobacco (Nicotiana tabacum) Recombinant ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA
Arachidonate 5-Lipoxygenase (ALOX5) protein (Myc-DYKDDDDK Tag)ALOX5 Origin: Human Host: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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- Application Notes
- Each Investigator should determine their own optimal working dilution for specific applications.
- Restrictions
- For Research Use only
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- Buffer
- 50 mM Tris containing 0 mM EDTA, pH 8.0.
- Handling Advice
- Avoid repeated freeze/thaw cycles.
- Storage
- -80 °C
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- Target
- ALOX5 (Arachidonate 5-Lipoxygenase (ALOX5))
- Alternative Name
- 5-Lipoxygenase (ALOX5 Products)
- Synonyms
- 5-LO Protein, 5-LOX Protein, 5LPG Protein, LOG5 Protein, 5LO Protein, 5LX Protein, AI850497 Protein, F730011J02 Protein, LOX5A Protein, si:dkey-194n13.2 Protein, lox5 Protein, arachidonate 5-lipoxygenase Protein, arachidonate 5-lipoxygenase a Protein, Arachidonate 5-lipoxygenase Protein, ALOX5 Protein, Alox5 Protein, alox5a Protein, AFUA_4G02770 Protein, AOR_1_1362014 Protein, lox5 Protein
- Background
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5-lipoxygenase (5-LO) catalyzes the formation of 5(S)-HpETE from arachidonic acid and its subsequent conversion to leukotriene A4. It is localized in the cytosol of resting human and rat peripheral blood neutrophils, whereas it is found in the nucleus in rat basophilic leukemia cells and human alveolar macrophages. Upon stimulation of the cell, 5-LO translocates to the nuclear membrane where it is found in association with 5-LO activating protein (FLAP) and with the 85 kDa cPLA2.
Alternative Names: 5-Lipoxygenase Antigen, 5 Lipoxygenase Recombinant Protein - Molecular Weight
- 78 kDa
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