NOG Protein
Quick Overview for NOG Protein (ABIN935687)
Target
See all NOG ProteinsProtein Type
Biological Activity
Origin
Source
Purity
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Sequence
- QHYLHIRPAP SDNLPLVDLI EHPDPIFDPK EKDLNETLLR SLLGGHYDPG FMATSPPEDR PGGGGGAAGG AEDLAELDQL LRQRPSGAMP SEIKGLEFSE GLAQGKKQRL SKKLRRKLQM WLWSQTFCPV LYAWNDLGSR FWPRYVKVGS CFSKRSCSVP EGMVCKPSKS VHLTVLRWRC QRRGGQRCGW IPIQYPIISE CKCSC
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Characteristics
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Purified recombinant Human Noggin protein
Expression System: 293 cells
Bioactivity: Determined by its ability to inhibit 5.0 ng/mL of BMP-4 induced alkaline phosphatase production by ATDC chondrogenic cells. The expected ED50 for this effect is 2.0-3.0 ng/mL of Noggin. -
Endotoxin Level
- < 0.1 ng per µg (1 EU/µg).
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Noggin (NOG) (AA 20-232) (Active) protein
NOG Origin: Mouse Host: CHO Cells Recombinant > 95 % as analyzed by SDS-PAGE. Active
Noggin (NOG) (AA 28-232) (Active) proteinNOG Origin: Human Host: CHO Cells Recombinant > 95 % as analyzed by SDS-PAGE. Active
Noggin (NOG) (AA 28-232) proteinNOG Origin: Human Host: CHO Cells Recombinant > 95 % as determined by Tris-Bis PAGE
Noggin (NOG) protein (His tag)NOG Origin: Mouse Host: Human Cells Recombinant > 95 % as determined by reducing SDS-PAGE.
Noggin (NOG) protein (Fc Tag)NOG Origin: Human Host: HEK-293 Cells Recombinant Greater than 95 % as determined by reducing SDS-PAGE.
Noggin (NOG) (AA 28-232) protein (His tag)NOG Origin: Mouse Host: Escherichia coli (E. coli) Recombinant Greater than 95 % as determined by SDS-PAGE WB, ELISA
Noggin (NOG) (AA 28-232) protein (GST tag)NOG Origin: Human Host: Wheat germ Recombinant WB, ELISA, AA, AP
Noggin (NOG) (AA 28-232) protein (Fc Tag)NOG Origin: Human Host: HEK-293 Cells Recombinant >95 % as determined by SDS-PAGE.
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Application Notes
- Each Investigator should determine their own optimal working dilution for specific applications.
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Restrictions
- For Research Use only
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Format
- Lyophilized
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Reconstitution
- Reconstitute in water to a concentration of 0.1 - 1.0 mg/mL.
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Buffer
- Supplied lyophilized with no additives.
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Preservative
- Without preservative
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Handling Advice
- Avoid repeated freeze/thaw cycles.
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Storage
- 4 °C/-20 °C
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Storage Comment
- Store at 4 °C until reconstitution. Following reconstitution aliquot and freeze at -20 °C for long term storage.
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- NOG (Noggin (NOG))
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Alternative Name
- Noggin
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Background
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Noggin belongs to a group of diffusible proteins which bind to ligands of the TGF beta family and regulate their activity by inhibiting their access to signaling receptors. The interplay between TGF beta ligands and their natural antagonists has major biological significance during development processes, in which cellular response can vary considerably depending upon the local concentration of the signaling molecule. Noggin was originally identified as a BMP-4 antagonist whose action is critical for proper formation of the head and other dorsal structures. Consequently, Noggin has been shown to modulate the activities of other BMPs including BMP-2, -7, -13, and -14. Targeted deletion of Noggin in mice results in prenatal death and recessive phenotype displaying a severely malformed skeletal system.
Alternative Names: SYM1 protein, NOG protein, SYM 1 protein, SYNS 1 protein, Symphalangism 1 (proximal) protein, Synostoses (multiple) syndrome 1 protein, SYNS1 protein -
Molecular Weight
- 46 kDa
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Pathways
- Stem Cell Maintenance, Tube Formation
Target
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