PDGF-AA Homodimer (Active) Protein
Origin: Human
Host: Escherichia coli (E. coli)
Recombinant
> 98 % pure
Active
-
- Target
- PDGF-AA Homodimer
- Protein Type
- Recombinant
- Biological Activity
- Active
-
Origin
- Human
-
Source
- Escherichia coli (E. coli)
- Sequence
- SIEEAVPAVC KTRTVIYEIP RSQVDPTSAN FLIWPPCVEV KRCTGCCNTS SVKCQPSRVH HRSVKVAKVE YVRKKPKLKE VQVRLEEHLE CACATTSLNP DYREEDTGRP RESGKKRKRK RLKPT
- Characteristics
-
Purified recombinant Human PDGF AA protein
Expression System: E.coli
Bioactivity: The ED50 as determined by the dose-dependent stimulation of thymidine uptake by Balb/c 3T3 cells is ? 1 ng/mL, corresponding to a specific activity of ? 1 x 10^6 units/mg. - Purity
- > 98 % pure
- Endotoxin Level
- < 0.1 ng per µg (1 EU/µg).
-
PDGF-AA Homodimer (AA 87-196) protein (His tag)
Origin: Rat Host: Escherichia coli (E. coli) Recombinant Greater than 95 % as determined by SDS-PAGE WB, ELISA
-
- Application Notes
- Each Investigator should determine their own optimal working dilution for specific applications.
- Restrictions
- For Research Use only
-
- Format
- Lyophilized
- Reconstitution
- Reconstitute in 10 mM CH0 OOH to a concentration of 0.1 - 1.0 mg/mL.
- Buffer
- Supplied lyophilized with no additives.
- Preservative
- Without preservative
- Handling Advice
- Avoid repeated freeze/thaw cycles.
- Storage
- 4 °C/-20 °C
- Storage Comment
- Store at 4 °C until reconstitution. Following reconstitution aliquot and freeze at -20 °C for long term storage.
-
- Target
- PDGF-AA Homodimer
- Alternative Name
- PDGF AA
- Background
-
PDGFs are disulfide-linked dimers consisting of two 12.0-13.5 kDa polypeptide chains, designated PDGF-A and PDGF-B chains. The three naturally occurring PDGFs, PDGF-AA, PDGF-BB and PDGF-AB, are potent mitogens for a variety of cell types including smooth muscle cells, connective tissue cells, bone and cartilage cells, and some blood cells. The PDGFs are stored in platelet a-granules and are released upon platelet activation. The PDGFs are involved in a number of biological processes, including hyperplasia, chemotaxis, embryonic neuron development, and respiratory tubule epithelial cell development. Two distinct signaling receptors used by PDGFs have been identified and named PDGFR-a and PDGFR-beta. PDGFR-a is high-affinity receptor for each of the three PDGF forms.
Alternative Names: Platelet-Derived Growth Factor-AA protein, ODGF protein, GDGF protein, Glioma-derived growth factor protein, Osteosarcoma-derived Growth Factor protein - Molecular Weight
- 28.5 kDa
-
You are here:
