PDGF-AA Homodimer (Active) Protein

Catalog No. ABIN935704

Recombinant protein expressed in Escherichia coli (E. coli).

Quick Overview for PDGF-AA Homodimer (Active) Protein (ABIN935704)

Target: PDGF-AA Homodimer

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: Escherichia coli (E. coli)

Purity: > 98 % pure

Product Details

Sequence: SIEEAVPAVC KTRTVIYEIP RSQVDPTSAN FLIWPPCVEV KRCTGCCNTS SVKCQPSRVH HRSVKVAKVE YVRKKPKLKE VQVRLEEHLE CACATTSLNP DYREEDTGRP RESGKKRKRK RLKPT

Characteristics: Purified recombinant Human PDGF AA protein
Expression System: E.coli
Bioactivity: The ED50 as determined by the dose-dependent stimulation of thymidine uptake by Balb/c 3T3 cells is ? 1 ng/mL, corresponding to a specific activity of ? 1 x 10^6 units/mg.

Endotoxin Level: < 0.1 ng per µg (1 EU/µg).

Application Details

Application Notes: Each Investigator should determine their own optimal working dilution for specific applications.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Reconstitute in 10 mM CH0 OOH to a concentration of 0.1 - 1.0 mg/mL.

Buffer: Supplied lyophilized with no additives.

Preservative: Without preservative

Handling Advice: Avoid repeated freeze/thaw cycles.

Storage: 4 °C/-20 °C

Storage Comment: Store at 4 °C until reconstitution. Following reconstitution aliquot and freeze at -20 °C for long term storage.

Target Details

Target: PDGF-AA Homodimer

Alternative Name: PDGF AA

Background: PDGFs are disulfide-linked dimers consisting of two 12.0-13.5 kDa polypeptide chains, designated PDGF-A and PDGF-B chains. The three naturally occurring PDGFs, PDGF-AA, PDGF-BB and PDGF-AB, are potent mitogens for a variety of cell types including smooth muscle cells, connective tissue cells, bone and cartilage cells, and some blood cells. The PDGFs are stored in platelet a-granules and are released upon platelet activation. The PDGFs are involved in a number of biological processes, including hyperplasia, chemotaxis, embryonic neuron development, and respiratory tubule epithelial cell development. Two distinct signaling receptors used by PDGFs have been identified and named PDGFR-a and PDGFR-beta. PDGFR-a is high-affinity receptor for each of the three PDGF forms.
Alternative Names: Platelet-Derived Growth Factor-AA protein, ODGF protein, GDGF protein, Glioma-derived growth factor protein, Osteosarcoma-derived Growth Factor protein

Molecular Weight: 28.5 kDa