SCGF beta Protein

Catalog No. ABIN935787

This Recombinant SCGF beta protein is expressed in Escherichia coli (E. coli).

Quick Overview for SCGF beta Protein (ABIN935787)

Target: SCGF beta

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: Escherichia coli (E. coli)

Purity: > 98 % pure

Product Details

Sequence: MGHGARGAER EWEGGWGGAQ EEEREREALM LKHLQEALGL PAGRGDENPA GTVEGKEDWE MEEDQGEEEE EEATPTPSSG PSPSPTPEDI VTYILGRLAG LDAGLHQLHV RLHALDTRVV ELTQGLRQLR NAAGDTRDAV QALQEAQGRA EREHGRLEGC LKGLRLGHKC FLLSRDFEAQ PSASPHPLSP DQPNGGTLEN CVAQASDDGS WWDHDCQRRL YYVCEFPF

Characteristics: Purified recombinant Human SCGF beta protein
Expression System: E.coli
Bioactivity: Determined by its inhibitory effect on the proliferation of TF-1 cells previously grown in media containing GM-CSF. The ED50 for this effect was observed at 7 ng/mL.

Sterility: Sterile filtered

Endotoxin Level: < 0.1 ng per µg (1 EU/µg).

Application Details

Application Notes: Each Investigator should determine their own optimal working dilution for specific applications.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Reconstitute with water to a concentration of 5 g/mL.

Buffer: Lyophilized from sterile filtered 0.25X PBS with 1 mM DTT and no additives.

Preservative: Dithiothreitol (DTT)

Precaution of Use: This product contains Dithiothreitol: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze/thaw cycles.

Storage: 4 °C/-20 °C

Storage Comment: Store at 4 °C until reconstitution. Following reconstitution aliquot and freeze at -20 °C for long term storage.

Target Details

Target: SCGF beta

Background: SCGF a and beta are hematopoietic growth factor that exerts their activity at early stages of hematopoiesis. The SCGFs are non-glycosylated species-specific cytokines that can support growth of primitive hematopoietic cells, and in combination with EPO or GM-CSF, promote proliferation or erthroid or myeloid progenitors, respectively.
Alternative Names: Stem Cell Growth Factor-beta protein

Molecular Weight: 25 kDa