SCGF beta Protein
Origin: Human
Host: Escherichia coli (E. coli)
Recombinant
> 98 % pure
Active
-
- Target
- SCGF beta
- Protein Type
- Recombinant
- Biological Activity
- Active
- Origin
- Human
- Source
- Escherichia coli (E. coli)
- Sequence
- MGHGARGAER EWEGGWGGAQ EEEREREALM LKHLQEALGL PAGRGDENPA GTVEGKEDWE MEEDQGEEEE EEATPTPSSG PSPSPTPEDI VTYILGRLAG LDAGLHQLHV RLHALDTRVV ELTQGLRQLR NAAGDTRDAV QALQEAQGRA EREHGRLEGC LKGLRLGHKC FLLSRDFEAQ PSASPHPLSP DQPNGGTLEN CVAQASDDGS WWDHDCQRRL YYVCEFPF
- Characteristics
-
Purified recombinant Human SCGF beta protein
Expression System: E.coli
Bioactivity: Determined by its inhibitory effect on the proliferation of TF-1 cells previously grown in media containing GM-CSF. The ED50 for this effect was observed at 7 ng/mL. - Purity
- > 98 % pure
- Sterility
- Sterile filtered
- Endotoxin Level
- < 0.1 ng per µg (1 EU/µg).
-
- Application Notes
- Each Investigator should determine their own optimal working dilution for specific applications.
- Restrictions
- For Research Use only
-
- Format
- Lyophilized
- Reconstitution
- Reconstitute with water to a concentration of 5 g/mL.
- Buffer
- Lyophilized from sterile filtered 0.25X PBS with 1 mM DTT and no additives.
- Preservative
- Dithiothreitol (DTT)
- Precaution of Use
- This product contains Dithiothreitol: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.
- Handling Advice
- Avoid repeated freeze/thaw cycles.
- Storage
- 4 °C/-20 °C
- Storage Comment
- Store at 4 °C until reconstitution. Following reconstitution aliquot and freeze at -20 °C for long term storage.
-
- Target
- SCGF beta
- Background
-
SCGF a and beta are hematopoietic growth factor that exerts their activity at early stages of hematopoiesis. The SCGFs are non-glycosylated species-specific cytokines that can support growth of primitive hematopoietic cells, and in combination with EPO or GM-CSF, promote proliferation or erthroid or myeloid progenitors, respectively.
Alternative Names: Stem Cell Growth Factor-beta protein - Molecular Weight
- 25 kDa
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