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VEGF 165 (Active) Protein

Recombinant protein expressed in Escherichia coli (E. coli).
Catalog No. ABIN935908

Quick Overview for VEGF 165 (Active) Protein (ABIN935908)

Target

VEGF 165

Protein Type

Recombinant

Biological Activity

Active

Origin

  • 20
  • 2
  • 1
Human

Source

  • 9
  • 8
  • 2
  • 1
  • 1
  • 1
  • 1
Escherichia coli (E. coli)

Purity

> 98 % pure
  • Sequence

    MAPTTEGEQK AHEVVKFMDV YQRSYCRPIE TLVDIFQEYP DEIEYIFKPS CVPLMRCAGC CNDEALECVP TSESNVTMQI MRIKPHQSQH IGEMSFLQHS RCECRPKKDR TKPEKHCEPC SERRKHLFVQ DPQTCKCSCK NTDSRCKARQ LELNERTCRC DKPRR

    Characteristics

    Purified recombinant Human VEGF165 protein
    Expression System: E.coli
    Bioactivity: Determined by the dose-dependent stimulation of the proliferation of Human umbilical vein endothelial cells (HUVEC). The expected ED50 is 4.0-8.0 ng/mL.

    Endotoxin Level

    < 0.1 ng per µg (1 EU/µg).
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  • Application Notes

    Each Investigator should determine their own optimal working dilution for specific applications.

    Restrictions

    For Research Use only
  • Format

    Lyophilized

    Buffer

    Supplied as a lyophilized powder.

    Handling Advice

    Avoid repeated freeze/thaw cycles.

    Storage

    4 °C/-20 °C

    Storage Comment

    Store at 4 °C until reconstitution. Following reconstitution aliquot and freeze at -20 °C for long term storage.
  • Target

    VEGF 165

    Alternative Name

    VEGF165

    Background

    VEGF is a potent growth and angiogenic cytokine. It stimulates proliferation and survival of endothellial cells, and promotes angiogenesis and vascular permeability. Expressed in vascularized tissues, VEGF plays a prominent role in normal and pathological angiogenesis. Substantial evidence implicates VEGF in the induction of tumor metastasis and intra-ocular neovascular syndromes. VEGF signals through the three receptors, fms-like tyrosine kinase(flt-1), kDaR gene product (the Mouse homolog of kDaR is the flk-1 gene product) and the flt4 gene product.
    Alternative Names: Folliculostellate cell-derived growth factor protein, Glioma-derived endothelial cell mitogen protein, VEGF-165 protein, Vascular Endothelial Growth Factor protein, VPF protein

    Molecular Weight

    38.5 kDa
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