WISP1 Protein

Catalog No. ABIN935909

This Recombinant WISP1 protein is produced in Escherichia coli (E. coli).

Quick Overview for WISP1 Protein (ABIN935909)

Target: WISP1 (WNT1 Inducible Signaling Pathway Protein 1 (WISP1))

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: Escherichia coli (E. coli)

Purity: > 98 % pure

Product Details

Sequence: TALSPAPTTM DFTPAPLEDT SSRPQFCKWP CECPPSPPRC PLGVSLITDG CECCKMCAQQ LGDNCTEAAI CDPHRGLYCD YSGDRPRYAI GVCAQVVGVG CVLDGVRYNN GQSFQPNCKY NCTCIDGAVG CTPLCLRVRP PRLWCPHPRR VSIPGHCCEQ WVCEDDAKRP RKTAPRDTGA FDAVGEVEAW HRNCIAYTSP WSPCSTSCGL GVSTRISNVN AQCWPEQESR LCNLRPCDVD IHTLIKAGKK CLAVYQPEAS MNFTLAGCIS TRSYQPKYCG VCMDNRCCIP YKSKTIDVSF QCPDGLGFSR QVLWINACFC NLSCRNPNDI FADLESYPDF SEIAN

Characteristics: Purified recombinant Human WISP1 protein
Expression System: E.coli
Bioactivity: The ED50 was determined by the dose-dependant proliferation of the MCF-7 cell line. The expected ED50 for this effect is 1.0-3.0 µg/mL.

Endotoxin Level: < 0.1 ng per µg (1 EU/µg).

Application Details

Application Notes: Each Investigator should determine their own optimal working dilution for specific applications.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Reconstitute in water to a concentration of 0.1 - 1.0 mg/mL (this product is slow to dissolve)

Buffer: Lyophilized from 10 mM CH3COOH.

Handling Advice: Avoid repeated freeze/thaw cycles.

Storage: 4 °C/-20 °C

Storage Comment: Store at 4 °C until reconstitution. Following reconstitution aliquot and freeze at -20 °C for long term storage.

Target Details

Target: WISP1 (WNT1 Inducible Signaling Pathway Protein 1 (WISP1))

Alternative Name: WISP1

Background: WISP-1 is a member of the CCN family of secreted cysteine rich regulatory proteins. It is expressed in the heart, kidney, lung, pancreas, placenta, ovary, small intestine and spleen. WISP-1 is a beta catenin regulated protein that can contribute to tumorigenesis and has also been shown to play a role in bone development and fracture repair. Human WISP-1 is a 38.1 kDa protein containing 346 amino acid residues. It is composed of four distinct structural domains (modules), the IGF binding protein (IGFBP) domain, the von Willebrand Factor C (VWFC) domain, the thrombospondin type-1 repeat (TSP type-1) domain, and a C-terminal cystine knot-like (CTCK) domain.
Alternative Names: WNT-1 inducible signaling pathway protein-1 protein, CCN4 protein, WISP-1 protein, WISP-1, WISP1, WISP 1 protein, Wnt-1-induced secreted protein protein, WISP-1 protein, WISP 1

Pathways: WNT Signaling, Growth Factor Binding