WISP1 Protein (AA 23-367) (His tag)

Catalog No. ABIN7275850

Product Details

Target: WISP1 (WNT1 Inducible Signaling Pathway Protein 1 (WISP1))

Protein Type: Recombinant

Protein Characteristics: AA 23-367

Origin: Mouse

Source: HEK-293 Cells

Purification tag / Conjugate: This WISP1 protein is labelled with His tag.

Purpose: Mouse WISP1 Protein

Sequence: Thr23-Asn367

Characteristics: Recombinant Mouse WISP1 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Thr23-Asn367.

Purity: > 95 % as determined by Tris-Bis PAGE

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1EU per μg by the LAL method.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.

Buffer: Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3-6 months after reconstitution.,2-8°C for 2-7 days after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Expiry Date: 12 months

Target Details

Target: WISP1 (WNT1 Inducible Signaling Pathway Protein 1 (WISP1))

Alternative Name: WISP1 (WISP1 Products)

Synonyms: CCN4 Protein, WISP1c Protein, WISP1i Protein, WISP1tc Protein, WISP-1 Protein, WISP1 Protein, si:ch211-38f10.2 Protein, AW146261 Protein, Elm1 Protein, zgc:172276 Protein, WNT1 inducible signaling pathway protein 1 Protein, WNT1 inducible signaling pathway protein 1a Protein, WNT1-inducible-signaling pathway protein 1-like Protein, WNT1 inducible signaling pathway protein 1b Protein, WISP1 Protein, wisp1a Protein, Wisp1 Protein, LOC100627044 Protein, wisp1b Protein

Background: The interplay between glioma stem cells (GSCs) and the tumor microenvironment plays crucial roles in promoting malignant growth of glioblastoma (GBM), the most lethal brain tumor. WISP1 is preferentially expressed and secreted by GSCs. Silencing WISP1 markedly disrupts GSC maintenance, reduces tumor-supportive TAMs (M2), and potently inhibits GBM growth. WISP1 signals through Integrin α6β1-Akt to maintain GSCs by an autocrine mechanism and M2 TAMs through a paracrine manner.

Molecular Weight: 39.4 kDa. Due to glycosylation, the protein migrates to 64-68 kDa based on Tris-Bis PAGE result.

UniProt: O54775

Pathways: WNT Signaling, Growth Factor Binding