Protein A Protein (HRP)

Catalog No. ABIN967967

This Native Protein A protein is produced in Staphylococcus aureus.

Quick Overview for Protein A Protein (HRP) (ABIN967967)

Target: Protein A

Protein Type: Native

Origin: Staphylococcus aureus

Source: Staphylococcus aureus

Application: Western Blotting (WB)

Product Details

Purification tag / Conjugate: This Protein A protein is labelled with HRP.

Characteristics: Protein A has been reported to bind strongly to rabbit IgG and mouse IgG2 isotypes. However, protein A does not bind mouse IgM and weakly binds IgG1. When conjugated to agarose, protein A acts as a solid matrix facilitating the immunoprecipitation of antigen-antibody complexes. It can also be utilized for antibody purification. As a horseradish peroxidase conjugate, protein A can be used instead of secondary antibody detection methods in Western blotting with polyclonal antibodies and with IgG2a and IgG2b mouse monoclonal antibodies.
Horseradish peroxidase (HRP) was coupled to protein A at a concentration of 4 mg/mg of protein A using the periodate method. For the detection of antigen using polyclonal antibodies in Western blotting, a concentration of 0.5 µg/ml (1:2000 dilution) is suggested.

Purification: Purified from tissue culture supernatant or ascites by affinity chromatography.

Application Details

Comment:

1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: 50 % glycerol, 20 mM sodium phosphate pH 7.5, 150 mM NaCl.

Storage: -20 °C

Storage Comment: Store undiluted at -20° C.

Target Details

Target: Protein A

Alternative Name: ProteinA